竹荪多糖提取工艺及其抗氧化性

以竹荪为原料,采用水提法提取竹荪多糖,比较了提取温度、pH、料液比和提取时间对竹荪多糖得率的影响,并且在单因素实验的基础上,采用正交实验,确定了水提法提取竹荪多糖的最佳工艺参数,即提取温度为90℃、pH为8.0、料液比为1:70 g/mL,时间为2.5 h。在上述提取条件下,提取竹荪多糖得率可达到13.09%。抗氧化实验结果表明,0.6 mg/mL的竹荪多糖对ABTS自由基的清除率可达92.76%,25 mg/mL的竹荪多糖对DPPH自由基的清除率可达70.55%。竹荪多糖提取工艺合理、可行,所提多糖具有较强的抗氧化性。

Extraction Technology of Dictyophora Polysaccharide and its Antioxidative Activity

Dictyophora polysaccharide from Dictyophora was extracted by hot water extraction. The effects of extraction temperature,pH,the powder-to-water ratio and extraction time were studied by single factor experiment and orthogonal experiment. The results showed that optimal extract was at temperature 90℃,pH8.0,powder-to-water ratio 1:70 g/mL and extraction in 2.5 h. Under this condition,the yield of Dictyophora polysaccharide was 13.09%. The results of anti-oxidation experiments of Dictyophora polysaccharide showed that the scavenging rate of ABTS free radicals by the 0.6 mg/mL Dictyophora polysaccharide was 92.76% and the scavenging rate of DPPH free radical by the 25 mg/mL Dictyophora polysaccharide was 70.55% respectively. The extraction technology is reasonable and feasible,and the polysaccharide has stronger antioxidant activity.