多酚氧化酶的提取及分离纯化研究进展

多酚氧化酶(PPO)是自然界中分布极广的一种金属蛋白酶,能催化邻-苯二酚氧化成邻-苯二醌,也是许多果蔬等农产品酶促褐变的主要原因。本文综述了国内外多酚氧化酶的提取及分离纯化技术的研究进展,包括传统提取方法(如缓冲液匀浆法、丙酮提取法、丙酮粉提取法等)和新型提取方法(超声波辅助提取法和超高压提取法等),传统经典分离纯化方法(如溶剂法、沉淀法)和新型分离纯化方法(如凝胶色谱分离法和离子交换色谱分离法等),旨在为多酚氧化酶的研究和应用,特别是为抑制农产品酶促褐变提供参考。

Progress in extraction and purification of polyphenol oxidase

Polyphenol oxidase(PPO)is a widely distributed in the nature of a metalloproteinase,it can catalyze the oxidation of o-benzenediol to o-benzodioxanone. And it is also the main reason for enzymatic browning of many fruits,vegetables and other agricultural products. In this paper,the recent advances in the extraction and purification of polyphenol oxidase from domestic and foreign were reviewed,including traditional extraction methods(such as buffer homogenization,acetone extraction,acetone powder extraction,etc.),new extraction methods(ultrasonic-assisted extraction and ultra-high pressure extraction,etc.),and also including the traditional classical separation and purification methods(such as solvent method,precipitation method),the new separation and purification methods(such as gel chromatography and ion exchange chromatography,etc.). The aim of this study is to provide reference for the research and application of polyphenol oxidase,especially for the inhibition of enzymatic browning in agricultural products.