翅果油联合有氧运动对高脂饮食小鼠血脂代谢紊乱及肝脏脂质积累的改善作用

目的:探讨翅果油联合有氧运动对高脂饮食小鼠血脂及肝脏脂质积累的影响。方法:将雄性C57BL/6小鼠,随机分为正常对照组(NC),高脂饮食组(HF),高脂饮食+翅果油灌胃组(HS),高脂饮食+有氧运动组(HE),高脂饮食+翅果油+有氧运动组(SE),每组8只,连续干预8周;喂养结束后采用酶学试剂盒测定小鼠血清甘油三脂(TG)、总胆固醇(TC)、低密度脂蛋白(LDL-C)、高密度脂蛋白(HDL-C)及肝脏TC、TG含量,并计算动脉粥样硬化指数(AI)和肝脏系数;采用H&E染色观察肝脏组织病理变化。结果:翅果油、有氧运动和翅果油联合有氧运动干预组小鼠体重,血清TG、TC、LDL-C,肝脏TC、TG,肝脏系数,AI值及肝脏组织脂肪病变程度均显著低于高脂饮食组(p<0.05),且翅果油联合有氧运动干预效果更好。结论:翅果油联合有氧运动能够显著改善由高脂饮食诱导的血脂代谢紊乱和肝脏脂质积累,降低动脉粥样硬化和脂肪肝的风险。

Samara Oil with Aerobic Exercise Ameliorates Lipid Metabolism Disorders and Hepatic Lipid Accumulation in High Fat Diet-fed Mice

Objective:To investigate the effect of samara oil with aerobic exercise on blood lipids and hepatic lipid accumulation in high fat diet-fed mice. Methods:The male C57BL/6 mice were randomly divided into normal diet-fed group(NC),high fat diet-fed(HF),high fat diet-fed with samara oil group(HS),high fat diet-fed with aerobic exercise group(HE),high fat diet-fed with samara oil and aerobic exercise group(SE). Eight mice for each group were treated for 8 weeks. After experiments,ELISA assays were applied to measure the plasma triglyceride(TG),total cholesterol(TC),low-density lipoprotein cholesterol(LDL-C),how-density lipoprotein cholesterol(HDL-C),and hepatic TC,TG. Atherosclerosis index(AI)and liver coefficient were calculated,and H&E staining was used to observe histopathological changes in liver tissues. Results:The body weight,TG,TC,LDL-C of serum,TG,TC of hepatic,AI and liver tissue fatty degeneration reduced significantly compared with HF group(p<0.05).Furthermore,the combined treatment of samara oil with aerobic exercise showed better effect. Conclusion:Samara oil with aerobic exercise could significantly improve lipid metabolism disorders and hepatic lipid accumulation induced by high fat diet feeding,and reduce the risk of atherosclerosis and fatty liver.