酸性电解水对副溶血性弧菌和单核细胞增生李斯特氏菌杀菌效果的比较研究

目的研究不同浓度酸性电解水对副溶血性弧菌和单核细胞增生李斯特氏菌单增李斯特菌的杀菌作用,比较酸性电解水对革兰氏阴性菌和革兰氏阳性菌的杀菌效果。方法采用传统的平板计数法进行细菌计数,扫描电镜观察细菌细胞的形态变化,琼脂糖胶电泳检测细菌DNA变化和BSA法进行细菌蛋白质泄露测定。结果酸性电解水对副溶血性弧菌和单核细胞增生李斯特氏菌均具有很强的杀灭效果,其杀菌效果随着电解水浓度的增加而增强。较高浓度的电解水处理后,副溶血性弧菌几乎全部杀灭,单核细胞增生李斯特氏菌菌落总数降低了1.45 log CFU/mL。扫描电镜实验表明,经酸性电解水处理的副溶血性弧菌坍塌明显,且随着酸性电解水浓度的升高,细胞形态崩解严重,细菌形状模糊。结论相较于单核细胞增生李斯特氏菌,酸性电解水对革兰氏阴性菌副溶血性弧菌有明显的杀菌效果

Bactericidal effect on Vibrio parahaemolyticus and Listeria monocytogenes by acidic electrolyzed water

Objective To study the bactericidal effect of different concentrations of acidic electrolyzed water on Vibrio parahaemolyticus and Listeria monocytogenes, and compare the bactericidal effect of acidic electrolyzed water on gram-negative and gram-positive bacteria. Methods Bacteria were counted by using the traditional plate counting method. The morphological changes of the bacterial cells were observed by scanning electron microscopy. Agarose gel electrophoresis was used to detect bacterial DNA changes and BSA method was used to measure bacterial protein leakage. Results Acidic electrolyzed water (AEW) had strong bactericidal activity against Vibrio parahaemolyticus and Listeria monocytogenes which became stronger with increasing AEW concentration. Vibrio parahaemolyticus was almost completely killed after treated with a higher concentration of electrolyzed water, and the number of Listeria monocytogenes strains decreased by 1.45 log CFU/mL. Scanning electron microscopy experiments showed collapsed significantly on Vibrio parahaemolyticus. The cell morphology was disintegrated seriously and the shape of the bacteria was blurred with increasing AEW concentration. Conclusion Compared to Listeria monocytogenes, acidic electrolyzed water has a significant bactericidal effect against Vibrio parahaemolyticus.