绿豆皮可溶性膳食纤维的抗氧化作用

通过体外、体内实验研究绿豆皮可溶性膳食纤维的抗氧化作用。结果表明：当质量浓度为4 mg/mL时， 绿豆皮可溶性膳食纤维的还原力为1.526，对1,1-二苯基-2-三硝基苯肼自由基和羟自由基的清除率分别为82.65% 和85.16%。动物实验结果显示，与正常对照组相比，D-半乳糖衰老模型组小鼠血清和肝组织中总抗氧化能力以 及过氧化氢酶、总超氧化物歧化酶、谷胱甘肽过氧化物酶活力均有所降低，丙二醛含量升高，且大部分差异显著 （P＜0.05，P＜0.01），说明小鼠D-半乳糖衰老模型构建成功。与模型组相比，绿豆皮可溶性膳食纤维各剂量组小 鼠血清和肝组织中丙二醛含量大幅降低，高剂量绿豆皮可溶性膳食纤维可显著提高小鼠血清和肝组织总抗氧化能力 以及过氧化氢酶、总超氧化物歧化酶、谷胱甘肽过氧化物酶活力（P＜0.05）。因此，绿豆皮可溶性膳食纤维具有 良好的抗氧化作用。

Antioxidant Effect of Soluble Dietary Fiber from Mung Bean (Phaseolus radiates L.) Hull

The in vivo and in vitro antioxidant effects of soluble dietary fiber (SDF) from mung bean (Phaseolus radiates L.) hull were studied. The results showed that the reducing power of SDF at a concentration of 4 mg/mL was 1.526, which could scavenge 82.65% 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and 85.16% hydroxyl radical. Animal experiments showed that total antioxidant capacity (T-AOC), catalase, total superoxide dismutase, glutathione peroxidase activity in the serum and liver tissue of D-Galactose-induced aging mice decreased, while malondialdehyde (MDA) content increased with significant differences being observed for most parameters (P < 0.05 or P < 0.01) when compared with the normal control group, indicating that the animal model of D-galactose-induced aging was constructed successfully. Compared with the model group, MDA content in the serum and liver tissue of mice administrated with SDF from all dose groups significantly decreased. High-dose SDF could significantly improve T-AOC and total superoxide dismutase, catalase, glutathione peroxidase activity in the serum and liver tissue of mice (P < 0.05). Therefore, SDF from mung bean hull has an excellent antioxidant activity.