| 一株拮抗海鱼干优势耐盐真菌芽孢杆菌HY-5筛选及其抗菌成分鉴定 |
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| 引用本文： | 杨秀文, 邓旗, 陶森, 孙力军, 王雅玲, 廖建萌, 刘颖, 房志家, 徐德峰. 一株拮抗海鱼干优势耐盐真菌芽孢杆菌HY-5筛选及其抗菌成分鉴定[J]. 食品工业科技, 2018, 39(16): 120-125. DOI: 10.13386/j.issn1002-0306.2018.16.022 |
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| 作者姓名： | 杨秀文 邓旗 陶森 孙力军 王雅玲 廖建萌 刘颖 房志家 徐德峰 |
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| 作者单位： | 1. 广东海洋大学食品科技学院, 广东省水产品加工与安全重点实验室, 广东湛江 524088;2. 湛江市食品药品检验所, 广东湛江 524000 |
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| 基金项目： | 广东省科技厅公益研究与能力建设专项资金项目(2014B020204005)。 |
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| 摘 要： | 目的:开发新的海鱼干耐高盐优势真菌抑制剂。方法:取用湛江市特呈岛红树林海域的海水、海泥以及红树林的根、茎和叶的样本,利用琼脂扩散法和平板点种法筛选出一株强抗菌活性菌株HY-5,通过传统细胞形态观察并结合生理生化实验及16S rDNA序列进行分析鉴定;利用杯碟法测定上清液对17株霉变海鱼干优势真菌抗菌谱,发酵液酸沉后经LC-MS检测其抗菌成分。结果:HY-5被鉴定为解淀粉芽孢杆菌,其上清液对17株霉变海鱼干优势真菌均有明显抗菌作用;LC-MS检测到HY-5发酵液中含2组抗菌脂肽surfactin(994.6、1008.6、1022.5和1036.6)和iturin(1016.5、1030.5、1044.4和1058.3)组分。结论:海洋源解淀粉芽孢杆菌HY-5的粗提液中含有抗菌脂肽,其对霉变海鱼干优势真菌有较强的拮抗作用。
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| 关 键 词： | 海洋源解淀粉芽孢杆菌 真菌 海鱼干 脂肽 液相色谱-质谱法(LC-MS) |
| 收稿时间： | 2018-01-04 |
Identification of Antagonistic Bacillus HY-5 and Its Antimicrobial Compositions from Mangrove Against Specific Salt Resistance Fungi of Marine Dried Fish |
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| YANG Xiu-wen, DENG Qi, TAO Sen, SUN Li-jun, WANG Ya-ling, LIAO Jian-meng, LIU Ying, FANG Zhi-jia, XU DE-feng. Identification of Antagonistic Bacillus HY-5 and Its Antimicrobial Compositions from Mangrove Against Specific Salt Resistance Fungi of Marine Dried Fish[J]. Science and Technology of Food Industry, 2018, 39(16): 120-125. DOI: 10.13386/j.issn1002-0306.2018.16.022 |
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| Authors: | YANG Xiu-wen DENG Qi TAO Sen SUN Li-jun WANG Ya-ling LIAO Jian-meng LIU Ying FANG Zhi-jia XU DE-feng |
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| Affiliation: | 1. Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China;2. Zhanjiang Food and Drug Institute, Zhanjiang 524000, China |
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| Abstract: | Objective:To discover a new antagonistic bacillus aganist specific fungi possessed high salt resistance of marine dry fish. Method:A strain with high antimicrobial activity named HY-5 was screened from seawater,sea mud,leaves and bark from mangroves of Zhanjiang by agar diffusion method and plate method. HY-5 was identified as Bacillus amyloliquefaciens by traditional cell morphology,physiological,biochemical experiments and 16S rDNA sequence analysis. The supernatant of HY-5 fermentation with obvious antibacterial effect on 17 strains fungi which isolated from moldy marine dried fish was determined by cup-dish method. And the fermentation broth contained two groups of antimicrobial lipopeptide surfactin(994.6,1008.6,1022.5 and 1036.6)and iturin(1016.5,1030.5,1044.4 and 1058.3)by LC-MS method. Conclusion:The crude extract of Bacillus amyloliquefaciens HY-5 contained antimicrobial lipopeptides which had strong antagonistic effect on fungi isolated from the marine dry fish. |
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| Keywords: | marine Bacillus amyloliquefacien fungi marine dry fish lipopeptide liquid chromatography-mass spectrometry(LC-MS) |
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