| 正交设计法优化富硒发芽糙米中硒蛋白提取工艺 |
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| 引用本文： | 王键,何余堂,曹智皓,高惠颖,杜佳阳,李燕红.正交设计法优化富硒发芽糙米中硒蛋白提取工艺[J].食品安全质量检测技术,2018,9(6):1434-1439. |
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| 作者姓名： | 王键 何余堂 曹智皓 高惠颖 杜佳阳 李燕红 |
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| 作者单位： | 渤海大学食品科学与工程学院生鲜农产品贮藏加工及安全控制技术国家地方联合工程研究中心 |
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| 基金项目： | 辽宁省大学生创新创业训练计划项目(2017101670096) |
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| 摘 要： | 目的优化硒蛋白的分离提取技术。方法以富硒发芽糙米为原料,采用碱提酸沉法提取硒蛋白,研究NaOH溶液浓度、料液比、浸提时间、pH值、(NH_4)_2SO_4饱和度5个因素对硒蛋白产量的影响,并通过正交试验确定硒蛋白分离提取的最优工艺。结果硒蛋白分离提取的最佳条件为:NaOH溶液浓度0.12 mol/L、料液比1:10(g:mL)、浸提时间4 h、pH 4.5、(NH_4)_2SO_4饱和度50%。在此条件下,10 g富硒发芽糙米中硒蛋白产量为327.56 mg,即硒蛋白得率约为3.28%,硒含量为0.73μg,换算成硒得率为48%。结论本方法简便快捷且硒蛋白及硒得率较高,可作为富硒发芽糙米中硒蛋白的分离提取方法。
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| 关 键 词： | 富硒 发芽糙米 硒蛋白 分离提取 |
| 收稿时间： | 2018/1/30 0:00:00 |
| 修稿时间： | 2018/3/26 0:00:00 |
Optimization of selenoprotein extraction process from se-enriched germinated brown rice with orthogonal design |
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| WANG Jian,HE Yu-Tang,CAO Zhi-Hao,GAO Hui-Ying,DU Jia-Yang and LI Yan-Hong.Optimization of selenoprotein extraction process from se-enriched germinated brown rice with orthogonal design[J].Food Safety and Quality Detection Technology,2018,9(6):1434-1439. |
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| Authors: | WANG Jian HE Yu-Tang CAO Zhi-Hao GAO Hui-Ying DU Jia-Yang and LI Yan-Hong |
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| Affiliation: | College of Food Science and Technology, Bohai University; National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products,College of Food Science and Technology, Bohai University; National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products,College of Food Science and Technology, Bohai University; National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products,College of Food Science and Technology, Bohai University; National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products,College of Food Science and Technology, Bohai University; National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products and College of Food Science and Technology, Bohai University; National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products |
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| Abstract: | |
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| Keywords: | rich selenium germinated brown rice selenoprotein separation and extraction |
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