| 微囊化Penicillium purpurogenum Li-3细胞的制备及其催化性能研究 |
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| 引用本文： | 黄晓林,曹红,欧阳巧凤,沈瑞麟,李春,吴廷华.微囊化Penicillium purpurogenum Li-3细胞的制备及其催化性能研究[J].食品工业科技,2018,39(18):156-161,169. |
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| 作者姓名： | 黄晓林 曹红 欧阳巧凤 沈瑞麟 李春 吴廷华 |
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| 作者单位： | 1. 浙江师范大学物理化学研究所, 浙江金华 321004;2. 嘉兴学院生物与化学工程学院, 浙江嘉兴 314001;3. 石河子大学化学化工学院, 新疆石河子 832003;4. 北京理工大学生命科学与技术学院, 北京 100081 |
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| 基金项目： | 国家自然科学基金资助项目(21266029)浙江省自然科学基金(LY17B060011)。 |
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| 摘 要： | 采用海藻酸钠(SA)-羧甲基纤维素钠(CMC)液芯微胶囊技术制备微囊化产紫青霉细胞(Penicillium purpurogenum Li-3),研究其制备条件及其催化性能,考察不同因素对微囊化细胞直径、机械强度、破损率、催化活性的影响。结果表明,在羧甲基纤维素钠的浓度为1.4%、海藻酸钠的浓度为1.2%、CaCl2的浓度为1.0%、固化过程CaCl2浓度为0.5%及加菌量为3.0%的条件下,制得的微囊化细胞在重复利用9次后,相对活性仍达到56.9%,显示出较好的机械强度和操作稳定性。本文为高效生物转化甘草酸合成单葡萄糖醛酸基甘草次酸(GAMG)提供了技术支持。
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| 关 键 词： | 微囊化 产紫青霉细胞 甘草酸 单葡萄糖醛酸基甘草次酸 |
| 收稿时间： | 2018-01-04 |
Preparation and Catalytic Properties of Microencapsulated Penicillium purpurogenum Li-3 |
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| HUANG Xiao-lin,CAO Hong,OUYANG Qiao-feng,SHEN Rui-lin,LI Chun,WU Ting-hua.Preparation and Catalytic Properties of Microencapsulated Penicillium purpurogenum Li-3[J].Science and Technology of Food Industry,2018,39(18):156-161,169. |
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| Authors: | HUANG Xiao-lin CAO Hong OUYANG Qiao-feng SHEN Rui-lin LI Chun WU Ting-hua |
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| Affiliation: | 1. Institute of Physical Chemistry, Zhejiang Normal University, Jinhua 321004, China;2. College of Biological Chemical Sciences and Engineering, Jiaxing University, Jiaxing 314001, China;3. School of Chemistry and Chemical Engineering, Shihezi University, Shihezi 832003, China;4. School of Life Science and Technology, Beijing Institute of Technology, Beijing 100081, China |
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| Abstract: | The microencapsulated Penicillium purpurogenum Li-3 cells were prepared with the entrapment technique of liquid-core microcapsules from sodium alginate (SA) and sodium carboxymethyl cellulose (CMC) in this paper. The preparation conditions and catalytic performances of the microencapsulated cells were studied, and the effects of different factors on the diameter, mechanical strength, breakage rate and catalytic performance of microencapsulated cells were investigated. The optimal conditions of concentration of sodium carboxymethyl cellulose 1.4%, sodium alginate 1.2%, the CaCl2 1.0%, the CaCl2 0.5% in solidification process, and the amount of bacteria 3% were obtained for microencapsulated cells. And after continuous use for 9 batch cycles, 56.9% residual activity of the microencapsulated cells remained. The results suggested that the microencapsulated cells possessed comparatively high mechanical strength and stability. This paper provides technical support for high efficiency bioconversion of glycyrrhizin to glycyrrhetinic acid monoglyceric acid (GAMG). |
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