不同花色苷代表性成分的分离鉴定及体外抗氧化活性

本实验以紫甘薯、黑枸杞、黑加仑和桑葚花色苷提取物为原料,制备其单体花色苷组分并研究其体外抗氧化性质。选取每种花色苷中含量较高,分子量居中,具有代表性的单体化合物作为目标组分,采用高速逆流色谱制备分离四种来源的花色苷。选用甲基叔丁基醚-正丁醇-乙腈-水-三氟乙酸作为溶剂体系,流速设定为5 mL/min,转速为850 r/min,分离得到高纯度花色苷单体化合物。采用分光光度法、HPLC-MS法分析测定花色苷含量及主要组成,用DPPH自由基、羟自由基清除能力和总还原力的测定分析其体外抗氧化活性。结果表明,四种来源的花色苷中代表性的成分依次为芍药素-3-咖啡酰-阿魏酰槐糖苷-5-葡萄糖苷、矮牵牛素-3-O-对香豆酰芸香糖苷-5-O-葡萄糖苷、矢车菊-3-芸香糖苷和矢车菊-3-O-葡萄糖苷,它们均具有良好的体外抗氧化活性。

The isolation and identification of representative components of different anthocyanins and antioxidant activity

In this experiment,four anthocyanins were prepared by high-speed countercurrent chromatography using purple sweet potato,Lycium ruthenicum Murr.,black currant and mulberry for anthocyanin extract. Representative monomeric compounds extracted from the four samples were selected with more contents and medium molecular weights. The anthocyanin monomer compound of high purity was isolated by using methyl tert-butyl ether-n-butanol-acetonitrile-water-trifluoroacetic acid as solvent system. The flow rate was set at 5 mL/min and the rotation speed was 850 r/min. The content of anthocyanins and the main components were determined by spectrophotometry and HPLC-MS. The antioxidant activity was determined by DPPH radical,hydroxyl radical scavenging ability and total reducing power. The results showed that the representative components the four anthocyanin extracts were Peonidin-3-caffeoylferuloylsophoroside-5-glucoside,petunidin-3-O-(6-O-p-coumaryl)-rutinoside-5-O-glucoside,Cyanidin 3-rutinoside and Cyanidin——3-O-glucoside,respectively with good antioxidant activity in vitro.