蒲公英萜醇对人乳腺癌细胞MCF-7增殖及凋亡的影响

目的：探讨蒲公英萜醇对人乳腺癌细胞MCF-7增殖、凋亡的影响及其可能的分子机制。方法：体外培养人 乳腺癌细胞MCF-7，采用噻唑蓝比色法和平板克隆实验检测蒲公英萜醇对MCF-7细胞增殖的影响；Hoechst 33342荧 光染色法检测细胞凋亡的形态变化；Western blot法检测凋亡相关蛋白的表达。结果：蒲公英萜醇对人乳腺癌细胞 MCF-7有明显的增殖抑制作用（P＜0.05）；Hoechst染色结果显示，给药后细胞核内染色质凝集，核固缩，可见凋 亡小体；Western blot结果显示，蒲公英萜醇可极显著提高Bax/Bcl-2的比值（P＜0.01）以及上调活化型Caspase-3/9 的表达（P＜0.01），下调PARP的表达（P＜0.01）。结论：蒲公英萜醇能够明显抑制人乳腺癌细胞MCF-7增殖， 并通过线粒体途径诱导其凋亡。

Effect of Taraxerol on the Proliferation and Apoptosis of MCF-7 Human Breast Cancer Cells

Objective: To investigate the effect of taraxerol on the proliferation and apoptosis of breast cancer MCF-7 cells and to explore its possible mechanism of action. Methods: The effect of taraxerol on the proliferation of MCF-7 cells was detected by methylthiazolyldiphenyltetrazolium bromide assay and colony formation assay; cell apoptosis was tested by Hoechst 33342 fluorescence staining, and apoptosis-related protein expression was detected by Western blot. Results: Taraxerol significantly inhibited the proliferation of MCF-7 cells (P < 0.05). Hoechst staining revealed a series of morphological changes in MCF-7 cells associated with apoptosis, and Western blot data indicated that taraxerol significantly increased the ratio of Bax/Bcl-2 (P < 0.01) and up-regulated cleaved caspase-3/9 expression (P < 0.01), and down-regulated PARP expression (P < 0.01). Conclusion: Taraxerol can inhibit the proliferation, and induce the apoptosis of MCF-7 cells via the mitochondrial signaling pathway.