| 猪肺血管紧张素转化酶纯化工艺中试放大研究 |
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| 引用本文： | 廖丹葵,熊珍爱,周利琴,孙丽霞,伍善广,兰雄雕.猪肺血管紧张素转化酶纯化工艺中试放大研究[J].食品工业科技,2018,39(12):185-188. |
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| 作者姓名： | 廖丹葵 熊珍爱 周利琴 孙丽霞 伍善广 兰雄雕 |
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| 作者单位： | 1. 广西大学化学化工学院, 广西南宁 530004;2. 广西大学轻工与食品工程学院, 广西南宁 530004;3. 广西科技大学医学院, 广西柳州 545006 |
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| 基金项目： | 广西自然科学基金(2017GXNSFAA198289)广西自然科学基金(2017GXNSFBA198215)国家自然科学基金(21766003)国家自然科学基金(51372043)广西教育厅项目(KY2016YB035)。广西自然科学基金(2016GXNSFAA380055) |
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| 摘 要： | 采用DEAE离子交换层析-超滤法纯化猪肺血管紧张素转化酶(angiotensin converting enzyme,ACE),在小试工艺条件的基础上对DEAE离子交换层析进行中试放大。以ACE比活及酶活回收率为指标,考察工艺放大后的纯化效果,并进一步完善纯化工艺条件。结果表明,DEAE离子交换层析柱放大15倍后,最终得到的ACE比活为(1.05±0.04)U/mg,达到电泳级纯,与小试工艺的纯化结果无明显差异,酶活回收率为34.6%±0.3%,高于小试工艺。由此认为,猪肺ACE提取放大纯化工艺是可行的,且可提高纯化效率,该工艺为更大规模的纯化ACE奠定了基础。
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| 关 键 词： | 猪肺 血管紧张素转化酶 纯化 中试放大 |
| 收稿时间： | 2017-09-27 |
Pilot-scale study on purification of angiotensin converting enzyme(ACE)from pig lung |
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| LIAO Dan-kui,XIONG Zhen-ai,ZHOU Li-qin,SUN Li-xia,WU Shan-guang,LAN Xiong-diao.Pilot-scale study on purification of angiotensin converting enzyme(ACE)from pig lung[J].Science and Technology of Food Industry,2018,39(12):185-188. |
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| Authors: | LIAO Dan-kui XIONG Zhen-ai ZHOU Li-qin SUN Li-xia WU Shan-guang LAN Xiong-diao |
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| Affiliation: | 1. School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China;2. Light industry and Food Engineering College, Guangxi University, Nanning 530004, China;3. Guangxi University of Science and Technology, Liuzhou 545006, China |
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| Abstract: | Angiotensin converting enzyme(ACE)from pig lung was purified by DEAE ion exchange chromatography-ultrafiltration,and the DEAE ion exchange chromatography was amplified on the basis of the small-scale process. The specific activity of ACE and the recovery of enzyme activity were taken as indexes to examine the purification effect of the scale-up process and to further improve the purification conditions. The results showed that after 15 times amplification of DEAE ion exchange chromatography,the specific activity of purified ACE was(1.05±0.04)U/mg,which reached the grade of electrophoresis pure. There had no significant difference with the purification results of the small-scale process. The recovery of enzyme activity was 34.6%±0.3%,that higher than the small-scale process. This study indicated the pilot-scale of the purification was feasible and could greatly increase the purification efficiency. The process would provide a foundation for a larger scale of ACE purification. |
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