UPLC-UV-QDa联用系统快速分析野生樱桃李叶化学成分

采用UPLC-UV-QDa联用系统测定了4种不同野生樱桃李叶中黄酮苷元的含量,并对醇提物成分进行快速分析。结果表明,槲皮素和山奈酚为野生樱桃李叶主要黄酮苷元。UPLC测定2种苷元可在7.0min内达到基线分离,线性关系良好,平均回收率分别为98.65%(RSD=1.08%,n=3),102.02%(RSD=1.17%,n=3),表明该方法快速、准确、重复性好。不同品种和生长环境的野生樱桃李叶中黄酮苷元含量有差异,但均以山奈酚为主。阳坡紫果叶山奈酚含量最高为(7.68±0.45)mg/g,沟谷红果叶最低为(4.59±0.32)mg/g,总黄酮含量变化幅度为16.21～33.25 mg/g。通过UPLC-UV-QDa联用系统快速鉴别,阳坡紫果叶醇提物主成分为1种单咖啡酰奎宁酸、4种槲皮素苷、7种山奈酚苷。

Quick determination of chemical constituents in Prunus cerasifera Leave by UPLC-UV-QDa

UPLC-UV-QDa technique, combined with the acidic hydrolysis experiment were applied to quantitative and qualitative analysis flavonoid aglycones and methanol extracts from leaves of four different kinds of Prunus cerasifera. The result showed that quercetin and kaempferol were well separated in 7.0 minutes and had good linear relationship, the average recoveries of two flavonoid aglycones were 98.65% and 102.02% with RSD 1.08%(n=3) and 1.17%(n=3), the method was proved to be fast, accurate and repeatable. There was some difference in the content of flavonoid aglycone from different kinds of Prunus cerasifera in different environment, two flavonoid aglycones were found in all kinds of leaves, while kaempferol was the main composition. The content of kaempferol was the highest in leaves of purple fruit in sunny slope up to (7.68±0.45) mg/g, and the lowest in leaves of red fruit in valleys with (4.59±0.32) mg/g, the content of total flavonoids were from 16.21 to 33.25 mg/g. Composition of extracts from leaves of purple fruit in sunny slope were rapidly identified by UPLC-UV-QDa technique, including one caffeoylquinic acid, four quercetin glycosides and seven kaempferol glycosides. The above results provide theoretical support and technical basis for the further exploitation and utilization of Prunus cerasifera.