南极磷虾粉脱脂前后胰蛋白酶降解产物的变化

采用测序级胰蛋白酶酶解南极磷虾冻虾粉和脱脂南极磷虾粉,然后利用液相色谱-飞行时间质谱(LC-Q-TOF)对酶解产物进行扫描分析,寻找有差异的肽段,并用三重四级杆质谱进行验证。结果表明,共鉴定出四条肽段在南极磷虾加工成脱脂虾粉过程中丢失,其中有两条肽段来源于南极磷虾的精氨酸激酶,它们的序列分别为EDMELQK、ASVHVDLPGWAK,另外两条肽段来源于南极磷虾的原肌球蛋白,它们的序列分别为DQVSEALLK、LQNAEGEVAALNR。本文为利用质谱技术分析南极磷虾多肽提供了一定的理论基础。

Changes of Degradation Produces by Trypsin before and after Degreasing of Antarctic Krill Prowder

Antarctic krill frozen powder and degreasing powder were digested by sequence-grade trypsin separately. The enzymatic hydrolysate were filtrated by ultrafiltrate membrane then detected by LC-Q-TOF. Differential peptides were found before and after degreasing process and verified by triple quadrupole mass spectrometer. Results showed that, four petides were identified and lost during the processing of Antarctic krill into defatied shrimp powder. Two petides were derived from the arginine kinase of Antarctic kill, they were EDMELQK, ASVHVDLPGWAK, DQVSEALLK and LQNAEGEVAALNR. The former two were from arginine kinase and the later two were from tropomyosin. This paper could provide theoretical basis for the application of mass spectrometry to analyze the peptides of Antarctic krill.