老山芹降血糖功能成分提取及活性研究

为增加老山芹资源的开发利用价值,以老山芹为原料,采用不同溶剂(不同浓度乙醇溶液、甲醇、乙酸乙酯)超声波辅助提取老山芹活性成分,以α-葡萄糖苷酶抑制率和α-淀粉酶抑制率为指标,确定适宜的提取溶剂,并通过单因素和响应面优化试验确定可保留老山芹降血糖功能成分的最佳提取条件。结果表明:适宜的提取溶剂为80%乙醇溶液,优化后的提取条件为超声温度49 ℃,超声时间114.5 min,料液比1:15.3 (g/mL),在此条件下得到老山芹提取液对α-葡萄糖苷酶抑制率为69.52%±1.13%,α-淀粉酶抑制率为56.38%±0.96%,二者的IC₅₀分别为0.169、0.339 mg/mL。老山芹可以作为具有降血糖功能的抑制α-葡萄糖苷酶和α-淀粉酶的物质来源。

Extraction and Activity of Hypoglycemic Functional Components from Heraclum dissectum

To increase the value of Heraclum dissectum resource development and utilization,Heraclum dissectum was used as raw materia. The raw material was treated with different solvents(different concentrations of ethanol,methanol or ethyl acetate)to determine the proper extraction solutionl and the appropriate extraction solvent was determined by the inhibitory rate of α-glucosidase and α-amylase were chosen as indicator. And determined the optimal extraction conditions for the hypoglycemic functional component of Heraclum dissectum by single factor and response surface methods. The results showed that the optimum extraction solution was 80% ethanol,and the optimal extraction conditions were as follows:ultrasonic temperature was 49 ℃,ultrasonic time was 114.5 min and solid-liquid ratio was 1:15.3 g/mL. Under this condition,the inhibitory rate of α-glucosidase reached 69.52%±1.13% and the inhibitory rate of α-amylase reached 56.38%±0.96%. The IC₅₀ were 0.169 and 0.339 mg/mL respectively. Heraclum dissectum would be capable as source of α-glucosidase and α-amylase inhibitory for hypoglycemic function.