近平滑假丝酵母补糖发酵及糖氮代谢对产甘露醇的影响

为了探究近平滑假丝酵母(Candida parapsilosis)生长及代谢过程中对碳源和氮源的需求,本文对发酵各阶段的碳源和氮源变化利用情况进行检测,并设定一个空白对照组和四个不同时期的补糖实验组进行研究,分别观测各实验组各时期生物量和甘露醇产量。结果表明,在72 h时进行补糖为最佳时期,甘露醇产量达到最大值为60.28 g/L,相比于空白对照组高出19.82%。通过补糖工艺的研究,在氮源和碳源耗尽时,补糖可以促进甘露醇的产生。本文为以后的工业化生产提供了理论依据。

Effect of oligosaccharide-adding fermentation and sugar-nitrogen metabolism on Candida parapsilosis on producing mannitol

In order to explore the demand for carbon and nitrogen sources of Candida parapsilosis during the growth and metabolism,the charge and utilization of carbon and nitrogen sources at different stages of fermentation were determined,and a blank control group and four different periods of glucose supplement test groups were set up. The biomass and mannitol yield of each experiment group were measured respectively. The research showed that it was the optimal time to adding oligosaccharide when fermented for 72 h,and the yield of mannitol reached the maximum value 60.28 g/L which increased by 19.82% compared with the control group. Through the study of the sugar supplement process,when the nitrogen and sugar source was used up,the addition of glucose can promote the production of mannitol. This study provided a theoretical basis for the future industrial production.