黄秋葵超微粉多糖提取工艺的优化及其抗氧化活性测定

以黄秋葵为原料,制备黄秋葵超微粉,利用响应面设计法优化黄秋葵超微粉多糖的提取工艺,并测定其对DPPH自由基、超氧阴离子自由基、羟自由基的清除能力和还原力。结果表明:最优工艺条件为料液比1:100(g/mL)、微波时间2 min、超声波时间14 min、超声波功率800 W,此条件下黄秋葵超微粉多糖的得率为27.68%±0.42%。黄秋葵超微粉多糖的体外抗氧化活性在四种体系中的IC₅₀值分别是1.53、4.12、6.38、2.49 mg/mL,具有较好的抗氧化活性。

Optimization of extraction technology and determination of antioxidant activity of polysaccharides from superfine powder of okra

The yield of polysaccharides in superfine powder that okra was prepared into was studied by ultrasonic-microwave assisted extraction method.The optimum process conditions were determined by response surface method.The antioxidant activity in vitro of polysaccharides extracted from superfine powder of okra was determined, including DPPH radical-scavenging, hydroxyl radical-scavenging,superoxide anion radical scavenging and reducing powers.The optimum extraction conditions were as follows:microwave time 2 min, ultrasonic time 14 min, and ultrasonic power 800 W. Under this optimized conditions, the experimental and predicted yield of polysaccharides was 27.68%±0.42%.The IC₅₀ for scavenging capacity of superfine powder of okra against DPPH·,O₂-·,·OH and reducing power were 1.53,4.12,6.38 and 2.49 mg/mL respectively.