辽宁地区绿豆品种SSR指纹图谱构建及品种鉴定

目的:构建辽宁地区绿豆品种的DNA指纹图谱,建立一种快速准确的品种鉴定方法。方法:利用15对多态性丰富的简单重复序列(simple sequence repeat,SSR)引物构建辽宁地区48份绿豆品种的指纹数据库及SSR指纹图谱。结果:15对引物共扩增出58个等位基因,每对引物检测到2~7个不等,平均为3.9,其中特异性条带13个,占总数的22.4%。多态信息含量介于0.114 5~0.776 4之间,平均为0.378 4。选择鉴别能力较强的5对引物组合,区分率为75%;15对引物组合,区分率为89.68%。构建了0/1形式的指纹数据库和SSR指纹图谱,除个别品种外均具有唯一性,可用于品种鉴定。结论:SSR指纹图谱的构建为绿豆品种鉴定提供了新的途径,为绿豆资源评价、保护及新品选育提供技术支撑和科学参考。

Establishment of Simple Sequence Repeat Fingerprint for Identification of Mungbean Varieties (Vigna radiate L.) Grown in Liaoning

Objective: This study aimed to establish a DNA fingerprint for rapid and accurate identification of mung bean varieties grown in Liaoning. Methods: A total of 48 mung bean samples from different varieties were used to construct a fingerprint database and a DNA fingerprint with 15 pairs of polymorphic simple sequence repeat (SSR) primers. Results: A total of 58 alleles were detected, 2–7 of which, with an average of 3.9, were detected with each pair of the primers used, and 13 specific bands were obtained, accounting for about 22.4% of the total. The polymorphic information content (PIC) varied from 0.114 5–0.776 4, with an average of 0.378 4. Five pairs of efficient primers were selected. The discrimination rate obtained with their combination was as high as 75%, while a discrimination rate of 89.68% was obtained with a combination of all 15 primer pairs. Based on these results, the DNA fingerprint database composed of the digits 0 and 1 and the SSR fingerprint were established, which allowed unequivocal?identification of all the varieties except a few unique ones. Conclusion: The SSR fingerprint presented in this study can open up a new way of identifying mung bean varieties and provide a basis for mung resource evaluation and protection as well as breeding of new varieties.