桂花多酚类化合物电喷雾萃取电离质谱分析

为建立桂花中多酚类化合物的电喷雾萃取电离质谱（extractive electrospray ionization-mass spectrometry，EESI-MS）分析法，以四季桂花为材料，采用高效液相色谱（high performance liquid chromatography，HPLC）法和EESI-MS测定了其醇提液中主要的多酚类化合物组成。结果表明：四季桂花中总酚和总黄酮含量分别为（133.38±0.12）mg/g和（72.49±0.09）mg/g。采用HPLC法从其醇提液中检测到了15?种多酚类化合物，其中杨梅素、桑黄素、芦丁、柚皮素和（＋）-儿茶素含量较为丰富。EESI-MS在负离子模式下从四季桂花醇提液中检测出了原儿茶酸、p-香豆酸、咖啡酸、表儿茶素和绿原酸5?种化合物；正离子模式下检测出了肉桂酸、桑黄素和杨梅素等3?种化合物。EESI-MS具有样品用量少、不需复杂分离预处理、分析速度快等优点，可用于食品中多酚类化合物的分析。

Analysis of Polyphenolic Compounds in Osmanthus fragrans Flowers by Extractive Electrospray Ionization-Mass Spectrometry

This study aimed to establish a method for the determination of polyphenols in Osmanthus fragrans flowers by extractive electrospray ionization-mass spectrometry (EESI-MS). The main polyphenolic compounds in the methanol extract of O. fragrans var. semperflorens flowers were determined by high performance liquid chromatography (HPLC) and EESI-MS. The results showed that the contents of total phenols and total flavonoids in the methanol extract were (133.38 ± 0.12) and (72.49 ± 0.09) mg/g, respectively. Fifteen polyphenolic compounds were detected by HPLC, with myricetin, morin, rutin, naringenin and (+)-catechin being abundant. p-Protocatechuic acid, coumaric acid, caffeic acid, and epicatechin were detected by EESI-MS in the negative ion mode while cinnamic acid, morin and myricetin were detected in the positive ion mode. The EESI-MS method in this study had the advantages of low sample consumption, no complex pretreatment and short analysis time and could be used for the analysis of polyphenols in food samples.