云南丽江和吉林靖宇11种越橘果实花色苷组分的分析

采用高效液相色谱法对云南丽江、吉林靖宇11种越橘果实花色苷组分进行测定。通过标准品的分析,建立了飞燕草素-3-半乳糖苷、飞燕草素-3-葡萄糖苷、矢车菊素-3-半乳糖苷、矢车菊素-3-葡萄糖苷、锦葵色素-3-半乳糖苷、锦葵色素-3-葡萄糖苷的回归方程,相关系数为0.9939~0.9968,精密度RSD为2.00%~3.73%,回收率在98.90%~100.99%,方法准确可行。通过6种标准品的对比,供试的15个样品中,所有越橘品种均有飞燕草素-3-半乳糖苷、矢车菊素-3-半乳糖苷、锦葵色素-3-半乳糖苷3种花色苷,飞燕草素-3-半乳糖苷平均含量为223.99 μg/g、锦葵色素-3-半乳糖苷平均含量为153.34 μg/g,二者占总花色苷的65%。采用欧氏距离聚类分析表明,集群1为飞燕草素-3-半乳糖苷含量较高的三种越橘,代表品种为丽江雷戈西、丽江奥尼尔、靖宇杜克;集群2为6种花色苷总含量较高的越橘品种,代表品种为丽江北陆与靖宇早蓝,说明飞燕草素-3-半乳糖苷含量与花色苷总含量是评价越橘花色苷特点的重要因子。

Analysis of Anthocyanins Components of 11 Blueberries in Yunnan Lijiang and Jilin Jingyu

The anthocyanins components of 11 blueberries in Yunnan Lijiang and Jilin Jingyu were determined by HPLC. The regression equation of the concentration of delphinidin-3-galactoside, delphinidin-3-glucoside, cyanidin-3-galactoside, cyanidin-3-glucoside, malvidin-3-galactoside and malvidin-3-glucoside were achieved by analysis of standard. The results showed that accuracy and feasibility of the correlation coefficients was 0.9939~0.9968, precision RSDs of 2.00%~3.73% (n=5) and the recovery rates of six anthocyanins were in the range of 98.90%~100.99%. Delphinidin-3-galactoside, cyaniding-3-galactoside, malvidin-3-galactoside were contained in all samples by the intercomparison of 6 standards in 15 samples. The average content of delphinidin-3-galactoside and malvidin-3-galactoside were 223.99 and 153.34 μg/g with 65% of the total anthocyanins. Cluster 1 showed the higher contents of delphinidin-3-galactoside was contained by Lijiang Legacy, Lijiang O'neal, Jingyu Duke and cluster 2 showed the higher content of total anthocyanins of Lijiang Northland, Jingyu Earliblue by the cluster analysis of euclidean distance. In addition, the contents of delphinidin-3-galactoside and total anthocyanins were the evaluated important factor of blueberries anthocyanins characteristics.