反溶剂法制备姜黄素-高粱醇溶蛋白复合颗粒及其特性分析

以姜黄素为芯材，高粱醇溶蛋白为壁材，采用反溶剂法制备包埋姜黄素的高粱醇溶蛋白复合颗粒（简称复合颗粒），并对其理化性质及稳定性进行研究。结果表明：姜黄素与高粱醇溶蛋白制备复合颗粒的最佳芯壁比（质量比）为1∶10，产品平均粒径为13.17?μm，电位为19.38?mV，得率为87.51%，包封率为62.61%，负载率为6.51%。相比于高粱醇溶蛋白颗粒，扫描电子显微镜显示：复合颗粒呈球形，但表面有多孔结构；红外光谱结果显示：复合颗粒位于1?534、1?655?cm－1等处的波峰均发生红移，α-螺旋含量减少，β-折叠含量增加。24?h紫外光照射后，高粱醇溶蛋白包埋的姜黄素光稳定性提高33%。复合颗粒的粒径和多分散性系数在30?d室内环境贮藏过程中均无明显变化，但在环境pH?5～6，颗粒易聚集。结果表明：高粱醇溶蛋白的包埋作用有益于提高姜黄素的稳定性，可为姜黄素和高粱醇溶蛋白的高值化利用提供理论依据。

Preparation and Characterization of Kafirin Microparticles Encapsulating Curcum by Anti-Solvent Method

The encapsulation of curcum in kafirin microparticles by anti-solvent method was investigated in this study, and the physicochemical properties and stability of the microparticles were characterized. The results showed that the optimal curcumin/kafirin ratio (m/m) ratio was 1:10. The average particle size and zeta potential of the microparticles obtained under this condition were respectively 13.17 μm and 19.38 mV; the production yield was 87.51%, the entrapment efficiency was 62.61%, and the loading efficiency was 6.51%. Scanning electron microscopy (SEM) showed that kafirin microparticles encapsulating curcum were uniform spheres with a porous surface. Fourier-transform infrared spectroscopy (FTIR) analysis indicated that the peaks at 1 534 and 1 655 cm-1 red shifted; furthermore, the alpha helix and beta-sheet content were respectively reduced and increased. The stability of kafirin microparticles encapsulating curcum was significantly improved after being exposed to UV radiation for 24 h, and no significant changes were observed in their sizes or polydispersity index (PDI) after 30-day storage under ambient conditions. However, the microparticles were easily gathered at pH 5–6. Therefore, the kafirin microparticles encapsulating curcum can improve the stability of curcum, which will provide a theoretical basis for the high-value utilization of kafirin and curcum.