海棠果实多酚提取物对胃癌细胞BGC-803的体外抑制活性

以‘富士’苹果为对照,研究了4种海棠果实提取物的总多酚含量和对人胃癌细胞BGC-803的体外抑制活性。用福林-酚法测定总多酚含量,采用高效液相色谱法测定单体酚种类和含量,用细胞活力检测试剂盒和倒置显微镜检测对人胃癌细胞BGC-803的抑制作用。结果表明:5种果实提取物总多酚含量变化范围为405.99~4663.30 mg GAE/100 g,海棠果实提取物总多酚含量均高于‘富士’苹果;5种果实多酚提取物对人胃癌细胞BGC-803的抑制作用(IC₅₀值)比较结果显示,红果肉的‘亚当’海棠(42.29 μg/mL)和‘红丽’海棠(64.67 μg/mL)优于黄果肉的‘海红果’海棠(1466 μg/mL)和西府海棠(2291 μg/mL),4种海棠均优于‘富士’苹果(3476 μg/mL);5种果实多酚提取物中单体酚主要有原花青素B₁、原花青素B₂、儿茶素、表儿茶素等;两种红果肉海棠(‘亚当’海棠、‘红丽’海棠)中,以矢车菊素半乳糖苷和低聚原花青素类为主的多酚类物质含量高于黄果肉的‘海红果’海棠、西府海棠和‘富士’苹果。

Inhibition Effect in Vitro of Crabapple Fruits Extracts on BGC-803 Gastric Cancer Cell Lines

Took Malus domestica ‘Fuji’ as control, the total phenolic contents (TPC) and in vitro inhibition effect (VIE) on human gastric BGC-803 cell lines of four crabapples fruits extracts were detected. TPC were determined using Folin-Ciocalteu reagent. Individual phenolic compounds were determined by high performance liquid chromatography (HPLC). Inhibition effect on human gastric BGC-803 cell lines was detected using Cell Counting Kit-8 (CCK-8) and inverted microscope. The results showed that the TPC range of five fruits extracts was 405.99~4663.30 mg GAE/100 g, in which the TPC of crabapples were higher than Malus domestica ‘Fuji’. The results of inhibitory effect (IC₅₀ value) of five fruits extracts on human gastric BGC-803 cell lines showed that red-fleshed Malus ‘Adams’ (42.29 μg/mL) and Malus ‘Red splendor’ (64.67 μg/mL) were better than yellow-fleshed Malus micromalus ‘Haihongguo’ (1466 μg/mL) and Malus micromalus (2291 μg/mL), and four crabapples were all better than Malus domestica ‘Fuji’ (3476 μg/mL). The individual phenolics of five extracts mainly contained procyanidins B₁, procyanidins B₂, (+) -catechin, and (-) -epicatechin. Malus ‘Adams’ and Malus ‘Red splendor’, which were varieties with red flesh, had a higher content of cyanidin-3-galactoside and oligo procyanidins than yellow-fleshed Malus micromalus ‘Haihongguo’, Malus micromalus, and Malus domestica ‘Fuji’.