薰衣草残渣中黄酮的超声辅助提取工艺及其抗氧化活性

以薰衣草水蒸汽蒸馏法提取精油后的残渣为研究对象,采用响应面实验法对薰衣草残渣中黄酮的超声辅助提取工艺进行优化,并通过总抗氧化能力、DPPH自由基清除能力、还原力和铁离子螯合能力评价黄酮纯化物的抗氧化能力。结果表明,黄酮最佳提取工艺条件为:提取时间为92 min,乙醇浓度为74%,提取温度为48℃,料液比(w/v)为1:10。在此条件下实际测定的黄酮提取率可达到(3.1125±0.2010) mg/g。黄酮纯化物总抗氧化能力最大达到86.32%±1.59%,DPPH自由基清除能力最大值为81.11%±1.36%,还原能力最大值为0.456±0.02,铁螯合能力最大值为76.25%±1.04%。结论:经优化的黄酮提取工艺稳定可行,黄酮纯化物具有较好的抗氧化活性,可为薰衣草的综合利用提供一定的科学依据。

Flavonoids extracted by ultrasonic assisted method from lavender residue and its antioxidant activity

The flavonoids were extracted from residue of lavender after essential oil extracted by water vapor distillation. Extracted process of flavonoids with ultrasonic assisted method was optimized by response surface experiment. The antioxidant ability of flavonoids extracts was investigated through total antioxidant capacity, DPPH radical scavenging ability, reducing power and chelating ability. The result showed that the optimum extraction condition for flavonoids was as follows:extracting time was 92 min, extraction temperature was 48℃, concentration of ethanol was 74%, and ratio of solid-liquid(w/v)was 1/10. Flavonoids content can reach to(3.1125±0.2010) mg/g. Total antioxidant capacity of flavonoids extracts reached to 86.32%±1.59%, DPPH radical scavenging capacity of flavonoids extract was 81.11%±1.36%, reducing power was 0.456±0.02, and chelating ability was 76.25%±1.04%. Conclusion:the optimum extraction condition of flavonoids from residue of lavender was stable and feasible. Moreover, the extracts also exhibited high antioxidant activity. This research could provide a certain scientific basis for the comprehensive utilization of lavender.