栉江珧粗多糖提取工艺优化、组成分析及其抗氧化活性研究

在单因素实验的基础上,采用响应面法优化栉江珧粗多糖的水浸提取工艺,并对优化后提取得到的多糖的组成及其抗氧化活性进行分析。结果表明:栉江珧粗多糖提取的最佳工艺条件为提取温度94 ℃、提取时间215 min、液固比34:1 (mL/g),在此条件下粗多糖得率为18.37%。栉江珧粗多糖中多糖含量为94.39%、蛋白质含量为2.8%、糖醛酸含量为0.63%、硫酸基含量为0.08%;采用红外光谱扫描和气相色谱分析粗多糖的结构和单糖组成,结果表明该多糖由葡萄糖组成。此外该粗多糖的清除超氧自由基和羟基自由基以及金属螯合力的半抑制浓度分别为0.599、1.01和0.29 mg/mL。研究结果可以为栉江珧粗多糖活性研究和功能性产品的开发提供理论基础。

Optimization of extraction technology,composition analysis and antioxidant activity of crude polysaccharides from Atrina pectinata

Based on single-factor experiments,response surface methodology(RSM)was employed to optimize the water extraction process of crude Atrina pectinata polysaccharides(CAPPS). The constitute and antioxidant activity of CAPPS obtained by the optimized process were analyzed. The optimal extraction conditions were found to be extraction duration of 215 min at 94 ℃ with a water/material ratio of 34:1 (mL/g). The yield of crude polysaccharides under the optimized conditions was 18.37%. The contents of polysaccharide,protein,uronic acid and sulfate were 94.39%,2.8%,0.63% and 0.08% respectively. Structure and monosaccharide composition of crude polysaccharide were determined by infrared multispectral scanner and chromatographic analysis. And the results showed that crude CAPPS was made of glucose. The results indicated that CAPPS has the eliminating activity on superoxide anion free radicals and hydroxyl radical,as well as metal-chelating ability,and IC₅₀(the half-inhibitory concentration)values were 0.599 mg/mL,1.01 mg/mL and 0.29 mg/mL respectively. The results in the current study would provide a theoretic basis for future studies on activity and development of functional products with CAPPS.