| 超高效液相色谱-质谱联用法检测鱼肉中23种性激素残留量 |
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| 引用本文： | 冯月超,贾丽,王建凤,范筱京,邵鹏,曹沅.超高效液相色谱-质谱联用法检测鱼肉中23种性激素残留量[J].食品安全质量检测技术,2018,9(16):4417-4426. |
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| 作者姓名： | 冯月超 贾丽 王建凤 范筱京 邵鹏 曹沅 |
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| 作者单位： | 北京市理化分析测试中心北京市食品安全分析测试工程技术研究中心 |
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| 摘 要： | 目的采用QuEChERS快速前处理技术,建立鱼肉中23种性激素的超高效液相色谱-串联四极杆质谱(ultra high performance liquid chromatography tandem quadrupole mass spectrometry,UPLC-MS/MS)分析方法。方法鱼肉样品经乙腈提取后,采用QuEChERS快速方法提取、净化,浓缩后的目标物均采用BEH C_(18)分离柱、多反应监测模式(multiple reaction monitoring,MRM)、外标法定量。雄激素和孕激素采用流动相0.1%甲酸水-甲醇分离,电喷雾正离子(electronic spray ion,ESI~+)扫描;雌激素采用乙腈-水为流动相,电喷雾负离子(ESI~-)扫描。结果 23种性激素标准曲线性良好,相关系数(r~2)均大于0.99。方法的回收率范围60.93%～102.1%,相对标准偏差(relative standard deviation,RSD,n=6)小于16.67%,检出限(limit of detection,LOD,S/N=3)为0.0015～0.47μg/kg。结论该方法简单、高效、可靠性强、灵敏度高,可用于鱼肉中的23种性激素的残留量检测。
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| 关 键 词： | QuEChERS 液相色谱-串联质谱法 雄激素 孕激素 雌激素 |
| 收稿时间： | 2018/5/21 0:00:00 |
| 修稿时间： | 2018/6/22 0:00:00 |
Determination of 23 kinds of sex hormones in fish using ultra performance liquid chromatography-tandem mass spectrometry |
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| FENG Yue-Chao,JIA Li,WANG Jian-Feng,FAN Xiao-Jing,SHAO Peng and CAO Yuan.Determination of 23 kinds of sex hormones in fish using ultra performance liquid chromatography-tandem mass spectrometry[J].Food Safety and Quality Detection Technology,2018,9(16):4417-4426. |
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| Authors: | FENG Yue-Chao JIA Li WANG Jian-Feng FAN Xiao-Jing SHAO Peng and CAO Yuan |
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| Affiliation: | Beijing Centre for Physical and Chemical Analysis,Beijing Engineering Research Center of Food Safety Analysis,Beijing Centre for Physical and Chemical Analysis,Beijing Engineering Research Center of Food Safety Analysis,Beijing Centre for Physical and Chemical Analysis,Beijing Engineering Research Center of Food Safety Analysis,Beijing Centre for Physical and Chemical Analysis,Beijing Engineering Research Center of Food Safety Analysis,Beijing Centre for Physical and Chemical Analysis,Beijing Engineering Research Center of Food Safety Analysis and Beijing Centre for Physical and Chemical Analysis,Beijing Engineering Research Center of Food Safety Analysis |
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| Abstract: | |
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| Keywords: | QuEChERS liquid chromatography-tandem mass spectrometry androgens progesterones estrogens |
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