河蚬多肽螯合亚铁的制备工艺研究

以河蚬肉为原料制备河蚬多肽,探讨河蚬多肽与亚铁离子螯合的最佳工艺条件。在单因素实验的基础上,采用响应面分析法对河蚬多肽螯合亚铁离子的工艺条件进行优化,确定最佳的螯合工艺条件为pH6.0,多肽与亚铁离子质量比1.5:1,螯合时间41 min,螯合温度31℃,螯合率预测值为82.40%,实测值为81.63%。红外光谱分析表明,多肽中的氨基和羧基均参与了螯合反应。本研究拓宽了河蚬的加工利用途径,并为多肽螯合亚铁的开发应用提供理论基础。

Study on the technology of peptides from Corbicula fluminea meat chelated ferrous ions

Taking Corbicula fluminea meat as materials,the optimal conditions of peptides from Corbicula fluminea meat chelated with ferrous ions were researched. On the basis of single factor experiment,the best chelation technology of Corbicula fluminea peptides were optimized by response surface methodology. The optimal chelation parameter were as follows:pH6.0,Corbicula fluminea peptides/ferrous ions ratio(1.5:1,mg/mg),reaction time 41 minute and reaction temperature 31℃,and under these conditions,the predicted chelation rate was 82.40% while the actual chelation rate was 81.63%.Both amino and carboxyl groups were involved in the complex by infrared spectroscopic analysis. The research would wide the way to process and utilize Corbicula fluminea,and provide a certain theoretical basis for the development and application of peptides chelated ferrous ions.