轮叶党参粗多糖对体外培养小鼠脾淋巴细胞及RAW 264.7细胞的免疫活性

目的:研究轮叶党参多糖对小鼠脾淋巴细胞及巨噬细胞(RAW 264.7)作用以探究其免疫增强的功效。方法:浓度为1000,500,250,125 μg/mL多糖体外分别与脾脏细胞、RAW 264.7共培养,采用ELISA法检测细胞上清中细胞因子IL-2、IFN-γ、IL-6和TNF-α浓度的变化。采用MTT法,研究轮叶党参粗多糖对RAW 264.7增殖的影响。Griess法测定细胞上清液中NO的含量变化。通过RT-PCR法检测iNOS mRNA的生成情况。结果:与正常组相比,轮叶党参粗多糖组IL-2、IFN-γ、IL-6、TNF-α和NO的分泌量极显著增加(p<0.01),显著促进RAW 264.7增殖(p<0.05)。与正常组比较,iNOS mRNA明显增加。结论:轮叶党参粗多糖可与ConA协同增强T细胞活性,也可参与活化单核-巨噬细胞对特异性免疫与非特异性免疫活性起到正向调节的作用。

Effect of polysaccharide from codonopsis lanceolata[Codonopsis lanceolata(Sieb.et Zucc.)Trauv.] on immunomodulatory activity in mouse spleen lymphocytes and RAW 264.7

Objective:The effect of immune enhancecement was researched on the effect of Codonopsis lanceolata polysaccharide on mouse spleen lymphocytes and RAW 264.7 for exploring the effect of its immune enhancement. Methods:Conventional preparation of polysaccharide from Codonopsis lanceolata and mouse splenocytes or RAW 264.7 were respectively inoculated with the concentration of polysaccharides about 1000,500,250,125μg/mL. Adopted the ELISA method to exam the cell supernatant. Studied the change of IL-2,IFN-γ,IL-6,TNF-α concentration of cell factor secreted by codonopsis lanceolata polysaccharide in induced mouse splee n lymphocytes. Adopted MTT method to study the effect of polysaccharide on the proliferation of RAW 264.7. Measure the content changed of the NO by Griess method. The formation of iNOS mRNA was measured by RT-PCR. Results:Compared with the normal group,the crude polysaccharides of Codonopsis lanceolata significantly increased the secretion of IL-2,IFN-γ,IL-6,TNF-α and NO,and the difference was very significant(p<0.01). The proliferation of RAW 264.7 was significantly different(p<0.05). Compared with the normal group,iNOS mRNA increased significantly. Conclusion:Codonopsis Codonopsis lanceolata polysaccharide can enhance the activity of T cells synergistically with ConA,and can also participate in the activation of smononuclear macrophages which was a positive regulation of specific and non specific immune activities.