活性肽-N对D-半乳糖致衰老小鼠的作用效果

为了探讨一种新型抗衰老活性因子——活性肽-N食用后的抗衰老作用，本研究通过给小鼠连续皮下 注射D-半乳糖（200 mg/（kg·d），以体质量计，下同）建立衰老模型，造模的同时开始灌胃2.5、25.0、 125.0 μg/（kg·d）活性肽-N复乳，连续9 周。分别采用旷场实验和Morris水迷宫实验检测小鼠的运动探究能力和学 习记忆能力。上述行为学实验后，对小鼠血清中谷胱甘肽过氧化物酶（glutathione peroxidase，GSH-Px）活力、丙 二醛（malondialdehyde，MDA）含量及脑组织中的乙酰胆碱酯酶（acetyl cholinesterase，AChE）活力进行测定。结 果表明，与衰老模型组相比，活性肽-N复乳高剂量组能显著提高衰老小鼠的运动探究能力，改善其学习记忆能力， 提高血清中的GSH-Px活力28.38%（P＜0.05），降低MDA含量26.19%（P＜0.05），同时降低脑组织中的AChE活 力23.26%（P＜0.05）。综合结果表明该活性肽-N对D-半乳糖致衰老小鼠具有明显的抗衰老作用。

Protective Effect of Active Peptide-N on D-Galactose-Induced Aging in Mice

Herein, we evaluated the effect of active peptide-N as a new anti-aging agent on aging model mice. For this purpose, an aging model was established by subcutaneously injecting mice with D-galactose (200 mg/(kg·d)) once daily for nine weeks. Thereafter, the mice was separately orally administered with multiple emulsion containing active peptide-N at doses of 2.5, 25.0 and 125.0 μg/ (kg·d). The open field and the Morris water maze test were used to evaluate the locomotor activity and learning and memory ability of mice. Furthermore, glutathione peroxidase (GSH-Px) activity and malondialdehyde (MDA) content in the serum, and acetyl cholinesterase (AChE) activity in the brain tissue of mice were measured. The results showed that active peptide-N multiple emulsion could significantly improve the locomotor activity and learning and memory ability of D-galactose-induced aging mice. The activity of serum GSH-Px was improved by 28.38% (P < 0.05), the concentration of serum MDA was decreased by 26.19% (P < 0.05), and the brain AChE activity was increased by 23.26% in the treated groups (P < 0.05) compared with the aging model group. These results demonstrate that active peptide-N multiple emulsion has an obvious anti-aging effect in D-galactose-induced aging mice.