离子阱质谱测定脂质鉴别深海鱼油

建立基于高效液相色谱-离子阱质谱脂质识别技术，用于鉴别合成乙酯型和天然甘油酯型深海鱼油。使用反相液相色谱，C18色谱柱，异丙醇和乙腈梯度洗脱分离脂质。使用离子阱质谱数据依赖型二级扫描获得脂质多级碎裂指纹信息。通过总离子流、一级质谱、二级质谱离子树识别脂质指纹信息。识别获知二十二碳六烯酸（docose hexaenoie acid，DHA）和二十碳五烯酸（eicosapentaenoic acid，EPA）在乙酯型深海鱼油中存在方式为CH3CH2O-DHA和CH3CH2O-EPA，而在天然甘油酯型深海鱼油的存在方式为甘油三酯指纹信息。此方法能准确区分乙酯型深海鱼油和甘油酯型深海鱼油。

Detection of Deep Sea Fish Oil Adulteration by Lipid Analysis Using Ion Trap Mass Spectrometry

In this paper, a high performance liquid chromatography coupled to ion trap mass spectrometry (HPLC-ITMS) method was used to identify synthetic ester-type and natural glyceride-type deep sea fish oils by lipid analysis. Lipids were separated by reversed phase HPLC on a C18 column using gradient elution with isopropyl alcohol and acetonitrile. Multistage fragmentation fingerprints were acquired by ion trap mass spectrometry under a data-dependent scan mode. The fingerprint information was identified from the total ion current spectra, mass spectra and tandem mass spectra. It was found that docose hexaenoie acid (DHA) and eicosapentaenoic acid (EPA) existed in ester-type fish oil as CH3CH2O-DHA and CH3CH2O-EPA, respectively, while both existed as triglycerides in glyceride-type fish oil. This method can accurately distinguish between ethyl ester-type and glyceride-type deep sea fish oils.