柠檬烯对铜绿假单胞菌的抑菌活性及其机理

以铜绿假单胞菌为供试菌,研究了柠檬烯对铜绿假单胞菌的抑菌活性及机理。利用肉汤稀释法测定了最小抑菌浓度(MIC),评价了柠檬烯抑菌活性,通过细菌生长曲线的测定、扫描电镜观察、细胞膜通透性以及膜电位的测定,诠释柠檬烯对铜绿假单胞菌的抑菌机理。结果表明:柠檬烯能抑制铜绿假单胞菌的正常生长和增殖,最小抑菌浓度为10 mL/L;柠檬烯可以破坏细胞的正常形态,提高菌液的相对电导率,增加细胞膜的通透性;柠檬烯也能降低铜绿假单胞菌菌体的膜电位,干扰细胞正常代谢活动。柠檬烯通过破坏细菌细胞膜的方式,抑制铜绿假单胞菌生长,导致细菌细胞死亡。这说明柠檬烯可以改变铜绿假单胞菌细胞膜的通透性,破坏其正常细胞形态结构及完整性,从而有效地抑制铜绿假单胞菌的正常生长。

Antibacterial activity and mechanism of limonene against Pseudomonas aeruginosa

The antibacterial activity and mechanism of limonene against Pseudomonas aeruginosa were evaluated. The results showed that MIC of limonene on P. aeruginosa was 10 mL/L. Limonene inhibited the normal growth and proliferation of P. aeruginosa by measuring the bacterial growth curve. Limonene could destroy the normal morphology of cell, increase the relative conductivity of bacteria and the permeability of the cell membrane.Further,limonene reduced the membrane potential, interfered with normal metabolic activity of cell. By scanning electron microscopy, limonene destroyed cell membrane and inhibited the growth of P. aeruginosa,resulting in bacterial death.Limonene could change the permeability of the cell membrane of P. aeruginosa,destroy the normal cell morphology and integrity,thus effectively inhibit the normal growth.