魔芋粉对小鼠急性酒精性胃黏膜损伤和慢性酒精性肠损伤的影响

目的：研究魔芋粉对小鼠急性酒精性胃黏膜损伤和慢性酒精性肠损伤的影响，为开发天然的预防酒精性 脏器损伤的食品提供理论依据。方法：用乙醇体积分数56%的白酒灌胃造成急性酒精性胃黏膜损伤和慢性酒精性 肠损伤模型，并用不同剂量的魔芋粉（170、240、400 mg/kg mb·d）灌胃干预，设正常组和模型组。检测指标包 括胃黏膜溃疡指数、胃组织中超氧化物歧化酶（superoxide dismutase，SOD）活力和丙二醛（methane dicarboxylic aldehyde，MDA）、一氧化氮（nitric monoxide，NO）、前列腺素E2（prostaglandin E2，PGE2）的含量，盲肠内容 物中游离氨、短链脂肪酸（short-chain fatty acids，SCFA）、肠道微生物的含量，并观察胃、肠组织病理学切片。 结果：与模型组比较，魔芋粉中、高剂量组小鼠胃溃疡指数分别降低了25.42%和28.18%；MDA含量分别下降了 38.07%和47.32%；SOD活力和NO、PGE2、双歧杆菌、乳杆菌含量明显升高；盲肠的游离氨含量、肠球菌数量明显 降低，乙酸、正丁酸、戊酸含量升高，胃黏膜和肠道组织得到明显改善。结论：魔芋粉对小鼠急性酒精性胃黏膜损 伤与慢性酒精性肠损伤具有保护作用，其影响作用可能是通过降低胃黏膜脂质过氧化物产物含量，增强胃黏膜抗氧 化能力，改善肠道微生态，增强胃黏膜和肠道组织的屏障作用从而实现对慢性酒精性肠损伤的保护。

Protective Effect of Konjac Powder on Acute Alcoholic Gastric Mucosal Injury and Chronic Alcoholic Intestinal Injury in Mice

Objective: This work was designed to investigate the effect of konjac powder on acute gastric mucosal injury and chronic intestinal injury in mice in order to provide a theoretical basis for the development of natural food products against alcoholic organ damage. Methods: A mouse model of acute gastric mucosal injury and chronic intestinal injury was established by gavaging 56% (alcohol content by volume) Erguotou liquor, and then the mice were gavaged with different doses of konjac powder suspended in water. In addition, normal control and model groups were established in the experiment. The following indicators were measured: gastric mucosal ulcer index, the contents of SOD, MDA, NO and PGE2 in gastric tissue, the levels of free ammonia and short-chain fatty acids (SCFA) in cecum contents, and the intestinal microflora. Meanwhile, histopathological evaluation of the gastric mucosa and intestinal tissue was conducted. Results: Compared with the model group, gastric ulcer indexes in the middle-dose and high-dose treatment groups were reduced by 25.42% and 28.18%, while MDA levels were reduced by 38.07% and 47.32%, respectively. The levels of SOD, NO, PGE2, Bifidobacterium and Lactobacillus in the two dose groups were significantly increased; free ammonia and enterococcus in cecum contents were significantly decreased, whereas the levels of acetic acid, butyric acid and valproic acid were increased. Moreover, the pathological changes of the gastric mucosa and intestine were significantly improved. Conclusion: Konjac powder can execute a protective effect against alcoholic gastric mucosal injury and chronic alcoholic intestinal injury presumably by reducing gastric mucosal lipid peroxidation, increasing the antioxidant capacity of gastric mucosa, improving the intestinal microenvironment, and enhancing gastrointestinal mucosal barrier function.