利用黑曲霉B1401发酵废弃茶末浸提液产单宁酶的工艺优化

以废弃茶末为原料,接种黑曲霉,以液态摇瓶的形式进行单宁酶生产的研究。通过单因素实验、Plackett-Burman实验及Box-Behnken响应面实验优化黑曲霉B1401液态发酵产单宁酶的最佳工艺条件。结果表明,诱导物为6%单宁酸+3%茶多酚,碳源为复配碳源,即0.746%可溶性淀粉:0.254%蔗糖,氮源为复配氮源,即0.3%尿素:0.7%硝酸钠,接种量为1%,装液量为90 mL/250 mL,转速为140 r·min-1,温度为30 ℃,发酵时间为72 h,此条件下单宁酶酶活可达104.82 U/g。

Optimization of Tannase Production from Waste Tea Extract from Asp.niger B1401

Using waste tea residue as raw material and Aspergillus niger as fermentation starter,tannase was produced by liquid-state shake flask fermentation.The optimal conditions were determined by single factor experiments test,Plackett-Burman test and Box-Behnken response surface method.Results showed that the optimum conditions for the liquid-state fermentation by Aspergillus niger were:6% tannic acid:3% tea polyphenols as mixed inducer,0.746%soluble starch:0.254%sucrose as mixed carbon source,0.3%urea:0.7%sodium nitrate as mixed nitrogen source,inoculum size 1%,medium contents 90 mL/250 mL,rotating speeds 140 r·min-1,temperature 30 ℃,the fermentation time for 72 h,the tannase activity reached 104.82 U/g.