双孢菇菇柄多糖柱层析纯化及单糖组成

以双孢菇菇柄为试材,首先对经超声提取得到的菇柄多糖进行柱层析分离纯化,然后对柱层析得到的多糖组分别采用紫外光谱和柱层析进行纯度分析,并进行红外光谱分析和单糖组成分析。结果表明,脱蛋白双孢菇菇柄多糖经DEAE Sephadex A-25柱层析纯化共得到4个组分,收集其中两个较多的组分(蒸馏水和0.1 mol/L NaCl洗脱组分),经紫外光谱扫描无核酸和蛋白质,经Sephadex G-200柱层析鉴定均为单一峰。所得两种多糖组分经FT-IR红外光谱分析,均含有多糖特征吸收峰,且均为吡喃糖环β-异构体的多糖。菇柄蒸馏水洗脱多糖组分由葡萄糖、半乳糖、甘露糖、木糖、阿拉伯糖组成,0.1 mol/L NaCl洗脱组分是由葡萄糖、半乳糖、甘露糖、木糖、阿拉伯糖、鼠李糖、氨基葡萄糖、氨基半乳糖组成。

Column chromatographic purification and monosaccharide composition of polysaccharide extracted from Agaricus bisporus stipe

Using Agaricus bisporus stipe as material,firstly,the A. bisporus stipe polysaccharide was extracted by ultrasonic extraction,and purified using column chromatography. Then the purity of polysaccharides isolated by column chromatography was examined by UV spectrum and column chromatography. The infrared spectrum and monosaccharide composition of the isolated polysaccharides were also analyzed. Results showed that the extracted polysaccharide was divided into 4 components by DEAE Sephadex A-25 purification,and the components eluted by distilled water and 0.1 mol/L NaCl were collected. UV spectrum scan showed that there were no nucleic acids and proteins in both the elutes,and they were also showed a single peak using Sephadex G-200. Infrared spectrum showed the both components contained characteristic absorption peaks of polysaccharide,and they were β-pyranose polysaccharides. The polysaccharide eluted by distilled water was composed by glucose,galactose,mannose,xylose and arabinose. And the 0.1 mol/L NaCl eluting component consists of glucose,galactose,mannose,xylose and arabinose,rhamnose,glucosamine and galactosamine.