蒙古扁桃油对高脂血症大鼠肝脏保护作用

目的:探究蒙古扁桃油对实验高脂血症大鼠血脂水平及肝脏功能的影响。方法:80只雄性Wistar大鼠按体重均衡随机分成8组:空白对照组、蒙古扁桃油对照组、高脂模型组、蒙古扁桃油组(A、B、C、D)、橄榄油对照组,除空白对照组和蒙古扁桃油对照组给予基础饲料,其余组均供给高脂饲料。5周建立高脂血症模型,蒙古扁桃油对照组(按每公斤体重给予10 mL/kg蒙古扁桃油灌胃)、蒙古扁桃油组(A:10、B:7.5、C:5、D:2.5 mL/kg)、橄榄油对照组(10 mL/kg橄榄油),空白组与模型组灌胃给予生理盐水。5周后,采样计算肝脏指数,测定血清总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)和肝组织丙二醛(MDA)、超氧化物歧化酶(SOD)、还原型谷胱甘肽(GSH),拟合量效关系曲线,观察肝脏组织学病理变化。结果:10 mL/kg和7.5 mL/kg的蒙古扁桃油显著降低高脂血症大鼠血清总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)浓度(p < 0.05),不同剂量蒙古扁桃油均显著降低肝脏丙二醇(MDA)的含量(p < 0.05),10 mL/kg和2.5 mL/kg蒙古扁桃油显著提高肝脏超氧化物歧化酶(SOD)活性和谷胱甘肽(GSH)的含量(p < 0.05)。结论:蒙古扁桃油具有调血脂、提高肝脏抗氧化和保护肝脏组织的作用,且最佳剂量在2.5 mL/kg ≤ Y ≤ 10 mL/kg范围内。

Effect of Amygdalus mongolica Oil on the Liver and Mechanisms in Hyperlipidemic Rats

Objective:To explore the effect of Amygdalus mongolica oil on blood lipid and function of liver in hyperlipidemic rats.Methods:80 female wistar rate were randomly assigned into eight groups of 10 rats each:the normal control group, Amygdalus mongolica oil control group, hyperlipidemia group, Amygdalus mongolica oil group (A, B, C, D), olive oil control group.Fed with basic diet and high-fat diet respectively.Five weeks to establish model of the hyperlipidemic rats, Amygdalus mongolica oil control group (Amygdalus mongolica oil at the dosages of 10 mL/kg per kilograms of body weight gavage), Amygdalus mongolica oil group (A:10, B:7.5, C:5.0, D:2.5 mL/kg), olive oil control group (olive oil, 10 mL/kg), the control and model group were orally given saline.Administered five weeks, the liver of the rats were dissected for evaluating the liver index.The serum levels of TC, TG, HDL-C and LDL-C were determined MDA, SOD and GSH were detected in the liver homogenate, as well as the histopathological changes of the liver were observed. Results:The Amygdalus mongolica oil of 10 mL/kg and 7.5 mL/kg significantly reduced serum TC, LDL-C levels (p < 0.05). The oils also significantly reduced the content of MDA content (p < 0.05).10 mL/kg and 2.5 mL/kg markedly increased SOD and GSH activities in liver (p < 0.05). Conclusion:The oil of Amygdalus mongolica had an important role in modulating hyperlipidemia, improving antioxidant effect on the liver and preventing liver damage.The optimum dose was in the range of 2.5 mL/kg and 10 mL/kg.