桃金娘果实花色苷提取物对两种假单胞菌生物被膜的抑制

为研究桃金娘果实花色苷提取物对隆德假单胞菌(Pseudomonas lundensis)和荧光假单胞菌(Pseudomonas fluorescens)生物被膜的抑制作用,采用肉汤稀释法测定了桃金娘果实花色苷提取物对两种假单胞菌的最小抑菌浓度和最小杀菌浓度,在亚抑菌浓度下采用结晶紫微孔板染色法测定了桃金娘果实花色苷提取物对两种假单胞菌的抑制作用和粘附情况。结果表明,桃金娘果实花色苷提取物对两种假单胞菌均有一定的抑制作用,对隆德假单胞菌和荧光假单胞菌的最小抑菌浓度分别为1500和1000 mg/mL,在500 mg/mL的亚抑菌浓度下,桃金娘果实花色苷提取物对隆德假单胞菌和荧光假单胞菌生物被膜具有显著抑制效果,能够显著降低两种假单胞菌在接触面上的粘附量(p<0.05)。桃金娘果实花色苷提取物具有开发为隆德假单胞菌和荧光假单胞菌生物被膜抑制剂的潜在价值。

Antibiofilm activity of anthocyanin extracts from Rhodomyrtus tomentosa(Ait.)Hassk for two pseudomonas bacterial

The inhibition effect of anthocyanin extracts from Rhodomyrtus tomentosa(Ait.)Hassk on the biofilm of Pseudomonas lundensis and Pseudomonas fluorescens were researched in this study. The minimum inhibitory concentration(MIC)and the minimum bactericidal concentration(MBC)of the extracts on the two bacterial were determined firstly. Then,the biofilm inhibition effect was measured using a microtiter plate assay. The results showed that the anthocyanin extract showed a certain inhibitory effect on both Pseudomonas lundensis and Pseudomonas fluorescens,and the minimum inhibitory concentrations of Pseudomonas lundensis and Pseudomonas fluorescens were 1500 and 1000 mg/mL respectively. At a sub-inhibitory concentration,500 mg/mL of the anthocyanin extract inhibited the biofilm formation of Pseudomonas lundensis and Pseudomonas fluorescens significantly,and reduced bacterial adhesion on the contact surface for the two pseudomonas significantly(p<0.05). The anthocyanin extracts from Rhodomyrtus tomentosa(Ait.)Hassk showed potential value for development as a biofilm inhibitor of Pseudomonas lundensis and Pseudomonas fluorescens.