响应面法优化蛹虫草固态发酵产物虫草素与腺苷综合提取工艺

以大米、啤酒糟共培养的蛹虫草固态发酵产物为原料，采用浸提法综合提取虫草素与腺苷，并利用响应面法优化提取条件。通过单因素试验考察提取时间、乙醇溶液体积分数、液料比和提取温度对综合提取虫草素与腺苷得率的影响。在单因素试验基础上，利用Box-Behnken试验设计，对浸提工艺中各影响因素进行优化。结果表明，浸提法综合提取虫草素与腺苷的最适工艺条件为纯水提取、提取温度45?℃、液料比22∶1（mL/g）、提取时间87?min，在该条件下虫草素与腺苷的综合得率为2.491‰，与模型理论预测值相近，说明该模型回归性良好，试验的拟合程度高。蛹虫草固态发酵产物中富含虫草素、腺苷，具有一定开发价值。

Optimization of Simultaneous Extraction of Cordycepin and Adenosine Produced by Solid-State Fermentation with Cordyceps militaris by Response Surface Methodology

Cordycepin and adenosine were simultaneously extracted from the solid-state culture of Cordyceps militaris with rice and brewer’s spent grain (BSG) as co-substrates. The extraction process was optimized using one-factor-at-a time method and response surface methodology (RSM). The total yield of cordycepin and adenosine was investigated as a function of four variables including extraction time, ethanol concentration, solvent-to-solid ratio and temperature. The optimal extraction conditions were found to be extraction at 45 ℃ for 87 min with water as a solvent at a solvent-to-solid ratio of 22:1 (mL/g). Under these conditions, The maximum total yield (2.491‰) of cordycepin and adenosine was experimentally obtained, which agreed with the predicted value (2.487‰). Accordingly, the regression model developed showed excellent goodness of fit. The solid-state culture of Cordyceps militaris is a promising rich of cordycepin and adenosine.