凝胶渗透色谱净化-高效液相色谱法测定食品中9种工业染料的基质效应

建立凝胶渗透色谱净化-高效液相色谱法测定食品中9种工业染料的方法,通过标准加入法分析火腿肠、肉肠、猪肉、火锅底料4种食品中工业染料的基质效应,并比较基质标准曲线和溶剂标准曲线方法的回收率,探讨基质种类、基质浓度、工业染料浓度对基质效应的影响,寻找减弱基质效应的方法。结果表明:9种工业染料的基质标准曲线和溶剂标准曲线在0.25~4.0 μg/mL浓度范围内线性关系良好;4种食品对9种工业染料均存在不同程度的基质效应;基质种类、基质浓度、工业染料浓度均影响基质效应的强度;用乙酸乙酯-环己烷(1∶1,v/v)提取,凝胶净化方式净化样品可以在一定程度上减弱基质效应,但不能消除基质效应。

Matrix effects of 9 industrial dyes in foods by high performance liquid chromatography with gelpermeation chromatography purification

By gel permeation chromatography purification-high-performance liquid chromatography,the methods were built to measure 9 industrial dyes in foods. The matrix effects of 4 types of foods including ham,sausage,pork and hotpot condiment,were analyzed by standard addition method. The recovery rate were compared between matrix of standard curve and solvent standard curve methods in order to probe the effects of the matrix type,matrix concentration and the industrial dye concentration to matrix effects and find the methods to weaken matrix effects. The results showed that there were good linear relationship between the matrix standard curve and solvent standard curve of 9 industrial dyes in the range of 0.25~4.0 μg/mL. All four kinds of foods had matrix effects of different degrees for 4 types of foods to 9 industrial dyes. The matrix type,matrix concentration and the industrial dye concentration all affected the intensity of matrix effects. By ethyl acetate-cyclohexane(1∶1,v/v)extraction,purified samples extracted by gel permeation extraction weakened the matrix effects to some degree,but matrix effects could not be eliminated.