新疆零售牛肉中沙门菌毒力基因的检测

目的了解新疆库尔勒市零售牛肉沙门菌污染状况和沙门菌分离株毒力基因携带状况。方法参考食品安全国家标准GB 4789.4-2016的方法,采用培养基培养和沙门菌生化鉴定试剂盒对零售牛肉中的沙门菌进行分离鉴定,并对沙门菌分离株10种毒力基因(inv A、hil A、ssa Q、mgt C、sii D、sop B、spv B、spv C、spv D、spv R)进行PCR检测。结果 317份牛肉样品共检出沙门菌阳性样品28份,分离鉴定到阳性沙门菌28株,污染率为8.8%(28/317);除ssa Q、spv C毒力基因外,其余8种毒力基因均有检出。毒力基因inv A和spv R具有高度的稳定性,携带率均为100%(28/28);毒力基因sii D、spv B和spv D的携带率也较高,分别达78.6%(22/28)、75.0%(21/28)和75.0%(21/28)。结论新疆库尔勒市零售牛肉沙门菌污染较为严重,应采取措施加强对新疆库尔勒市牛肉生产各环节的防控与食品卫生监督。

Virulence gene detection of Salmonella from retail beef in Xinjiang

Objective To understand the status of Salmonella contamination and virulence gene carrying of retail beef in Korla, Xinjiang. Methods According to the national standard of food safety GB 4789.4-2016, isolation and identification of Salmonella in retail beef was carried out by using culture medium and salmonella biochemical identification kit, and ten virulence genes (invA, hilA, ssaQ, mgtC, siiD, sopB, spvB, spvC, spvD, and spvR) isolated from Salmonella were detected by PCR method. Results A total of 28 samples were tested positive for Salmonella in 317 beef samples, and 28 strains were isolated and identified with a contamination rate of 8.8% (28/317). Eight virulence genes were detected except virulence genes ssaQ and spvC. The virulence genes invA and spvR had high stability and the carrying rates were 100% (28/28). The carrying rates of virulent genes siiD, spvB and spvD were also higher, reaching 78.6% (22/28), 75.0% (21/28) and 75.0% (21/28), respectively. Conclusion The contamination of Salmonella in retail beef is very serious in Korla of Xinjiang. Measures should be taken to strengthen prevention and control of beef production and food hygiene supervision in Korla of Xinjiang.