Bio- and bioelectro-catalytic properties of polyaniline/poly(acrylic acid) composite films bearing covalently-immobilized acid phosphatase |
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| Affiliation: | 1. CSIRO Materials Science and Engineering, P.O. Box 21, Belmont, Victoria 3216, Australia;2. Institute for Frontier Materials, Deakin University, Geelong 3216, Australia;3. School of Fashion and Textiles, RMIT University, 25 Dawson Street, Brunswick, Victoria 3056, Australia;4. College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China;1. Faculdade de Odontologia, Universidade Federal Fluminense, Niterói, RJ, Brazil;2. Faculdade de Odontologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil;3. Laboratório de Instrumentação Nuclear, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil;4. Faculdade de Farmácia, Laboratório TIF, Universidade Federal do Rio de Janeiro - UFRJ, Rio de Janeiro, RJ, Brazil;5. Núcleo de Pesquisa em Biomateriais Dentários, Faculdade de Odontologia, Universidade Veiga de Almeida - UVA, Rio de Janeiro, RJ, Brazil;1. College of Chemistry and Bioengineering (Guangxi Key Laboratory of Environmental Engineering, Protection and Assessment), Guilin University of Technology, Guilin 541004, People’s Republic of China;2. Department of Chemistry, San José State University, One Washington Square, San José, CA 95192-0101, USA;1. Department of Food Engineering, Regional and Integrate University of Upper Uruguay and Missions, URI - Erechim, Av. Sete de Setembro 1621, Erechim, RS 99700-000, Brazil;2. Department of Chemical and Food Engineering, Federal University of Santa Catarina, UFSC, Campus Universitário, Bairro Trindade, Caixa Postal 476, Florianópolis, SC 88040-900, Brazil;3. Department of Food Engineering, Federal University of Santa Catarina UFSC, PO Box 476, 88040-900 Florianópolis, SC, Brazil |
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| Abstract: | Conducting films composed of polyaniline (PANI) and poly(acrylic acid) (PAA) were prepared by electrochemical polymerization of aniline in the presence of various concentrations of PAA. The content of PAA moiety on the surface of the composite films (PANI/PAA films) was estimated by determination of carboxyl groups and found to be controlled by the concentration of PAA in polymerization solution. Acid phosphatase (ACP) was immobilized covalently on the PANI/PAA films by the condensation reaction with the carboxyl groups on the films. It was confirmed that the enzyme activity of the ACP-immobilized PANI/PAA film increased with increasing content of PAA moiety on the surface of the film, accompanying an increase in the amount of the immobilized ACP. The activity of the covalently immobilized ACP was significantly higher than that of the ACP adsorbed on the PANI/PAA film. By use of the ACP-immobilized PANI/PAA film as an enzyme electrode, bioelectrocatalytic oxidation of L-ascorbic acid 2-phosphate (ASA2P) was examined. The enzyme electrode gave the current due to the oxidation of ASA2P in proportion to the content of PAA moiety on the surface of the PANI/PAA film used, which was relevant to the activity of the covalently immobilized ACP. |
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| Keywords: | Polyaniline Poly(acrylic acid) Acid phosphatase Enzyme electrode |
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