Immobilization of Aspergillus niger xylanase A on Fe3O4-coated chitosan magnetic nanoparticles for xylooligosaccharide preparation |
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| Affiliation: | 1. Unité Enzymes et Bioconversion, Ecole Nationale d’Ingénieurs de Sfax, Université de Sfax, route de Soukra 3038, Sfax, Tunisia;2. Unité de service commun bioréacteur couplé à un ultrafiltre, Ecole Nationale d’Ingénieurs de Sfax, Université de Sfax, route de Soukra 3038, Sfax, Tunisia;1. Department of Nanoscience and Technology, Bharathiar University, Coimbatore 641 046, India;2. Centre for Automotive Energy Materials, ARCI, Chennai 600 113, India;3. Materials Science Group, Indira Gandhi Centre for Atomic Research, Kalpakkam 603 102, India;1. Department of Food Engineering and Technology, Institute of Chemical Technology, Mumbai, India;2. State Research Institute of Genetics and Selection of Industrial Microorganisms, Moscow, Russian Federation, Russia;3. Department of Chemistry, School of Chemical Technology, Aalto University, Finland;4. Department of Biotechnology and Chemical Technology, School of Chemical Technology, Aalto University, Finland;5. Juva Truffle Center, Huttulantie Juva, Finland;1. National Centre for Catalysis Research and Department of Biotechnology, Indian Institute of Technology-Madras, Chennai, 600 036, India;2. National Centre for Catalysis Research and Department of Chemistry, Indian Institute of Technology-Madras, Chennai, 600 036, India;3. School of Chemical Engineering and Analytical Science, The University of Manchester, Manchester, M13 9PL, United Kingdom;4. Department of Chemical and Process Engineering, University of Surrey, Guildford, Surrey, GU2 7XH, United Kingdom;1. Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China;2. Shandong Provincial Key laboratory of Glycoscience and Glycoengineering, Ocean University of China, Qingdao 266003, China |
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| Abstract: | Aspergillus niger xylanase A (XylA) was immobilized onto Fe3O4-coated chitosan magnetic nanoparticles prepared by the layer-by-layer self-assembly approach. The Fe3O4-coated chitosan magnetic nanoparticles showed a high binding capacity of 162.2 mg ? g? 1-particles and a recovery activity of 56.5% for XylA. The immobilized XylA showed improved thermostability and storage stability compared with free XylA. The immobilized XylA retained 87.5% activity after seven successive reactions by magnetic separation. Xylotriose and xylohexaose were the main products released from birchwood xylan and wheat bran insoluble xylan by immobilized XylA, respectively. |
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