Regional proton nuclear magnetic resonance spectroscopy differentiates cortex and medulla in the isolated perfused rat kidney |
| |
Authors: | G J Cowin I A Leditschke S Crozier I M Brereton Z H Endre |
| |
Affiliation: | (1) Centre for Magnetic Resonance, University of Queensland, Brisbane, Australia;(2) Department of Medicine, University of Queensland, Royal Brisbane Hospital, Clinical Sciences Building, 4029 Brisbane, Australia |
| |
Abstract: | Volume-localized proton nuclear magnetic resonance spectroscopy was used as an assay of regional biochemistry in the isolated
perfused rat kidney. This model eliminated artifacts caused by respiratory and cardiac motion experiencedin vivo. Immersion of the kidney under its venous effluent reduced the susceptibility artifacts evoked by tissue-air interfaces.
The rapid acquisition with relaxation enhancement imaging sequence was used for scout imaging. This gave excellent spatial
resolution of the cortex, outer medulla, and inner medulla. Spectra were then acquired in 10 minutes using the volume-selective
multipulse spectroscopy sequence from voxels with a volume of approximately 24 μL located within the cortical or medullary
regions. Spectral peaks were assigned by the addition of known compounds to the perfusion medium and by comparison with spectra
of protein-free extracts of cortex and medulla. The medullary region spectra were characterized by signals from the osmolytes
betaine, glycerophosphorylcholine, and inositol. The spectra from the cortex were more complex and contained lesser contributions
from osmolytes. |
| |
Keywords: | localized nuclear magnetic resonance spectroscopy isolated perfused rat kidney renal osmolytes |
本文献已被 SpringerLink 等数据库收录! |
|