测量微量液体折射率的新方法

液体折射率的毛细管焦点测量法具有样品需要量极少和封闭测量的特点,在微量液体的折射率测量方面有重要的应用前景。为了提高毛细管焦点测量法的测量精度和改善测量的方便性,用内置CCD的电子目镜取代传统目镜,在计算机上直接观察和判断毛细管焦点的成像状态;用电动精密位移台取代传统的手动精密位移台;用新的测量系统对不同浓度的乙二醇水溶液做了折射率测量。结果表明,折射率的测量精度达到±0.0002;一次性测量样品需要量小于0.002mL。在毛细管焦点测量法中引入电子目镜和电动精密位移台,提高了微量液体折射率的测量精度。

Measuring Method for Refractive Index of Micro-Quantity Liquid

The method for measuring refractive index of micro-quantity liquid within a glass capillary has a promising application in virtue of minimal sample requirement and confinement of liquid in a capillary.In order to improve the measurement uncertainty and convenience,the traditional eyepiece is substituted by a CCD-containing electronic eyepiece.In addition,an electric precision displacement stage is used instead of the traditional manual precision displacement stage.Refractive indices of solutions of glycol with various concentrations are measured by the new measurement system in our experiment.The measurement uncertainty reaches to ±0.0002 and the required liquid quantity is less than 0.002 mL for a single measurement.In the method for measuring refractive index of micro-quantity liquid within a glass capillary,the introduction of electronic eyepiece and electric displacement stage improves the uncertainty of the measurement.