Validation of a Real-Time PCR On-Site Quantification Method for MON810 Maize |
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| Authors: | Stephen R. H. Langrell Theodore R. Allnutt Val��rie Laval Yves Bertheau Maria Pla Nina Papazova David Lee |
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| Affiliation: | (1) European Commission–Joint Research Centre, Institute for Prospective Technological Studies, Edificio EXPO, 41092 Seville, Spain;(2) Food and Environment Research Agency, Sand Hutton, York, YO41 1LZ, UK;(3) Institut National de la Recherche Agronomique, Route de Saint Cyr, 78026 Versailles Cedex, France;(4) Institut de Tecnologia Agroaliment?ria, Universitat de Girona, 17071 Girona, Spain;(5) Institute for Agricultural and Fisheries Research, Burg. Van Gansberghelaan 115, 9820 Merelbeke, Belgium;(6) National Institute of Agricultural Botany, Huntingdon Road, Cambridge, CB3 0LE, UK |
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| Abstract: | A rapid on-, or near-site, quantitative method for use as a pre-harvest predictive decision, or co-existence monitoring, tool for adventitious genetically modified (GM) presence has been developed. Based on a laboratory-based protocol for real-time (RT) quantification of the MON810 GM event in maize kernels, the duplex RT polymerase chain reaction method was constructed around the portable Cepheid SmartCyclerII platform, requiring only modest support infrastructure for field application. Validation through an international ring trial showed good compliance with minimum assay performance requirements as defined by the European Network of GMO Laboratories (RSDr = 18.5%; RSDR = 32.8; Bias = 26.7%). |
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