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Interaction study on DNA,single-wall carbon nanotubes and acridine orange |
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| Authors: | Yang Meng Fengyu Liu Jingfeng Han Shiguo Sun Jiangli Fan Fengling Song Xiaojun Peng |
| Affiliation: | 1. State Key Laboratory of Fine Chemicals, Dalian University of Technology, No. 2 Linggong Road, High-tech District, Dalian 116024, China;2. School of Chemistry, Dalian University of Technology, 2 Linggong Road, Ganjingzi District, Dalian 116023, China;3. Division of Solar Energy, Dalian National Laboratory for Clean Energy, Dalian Institute of Chemical Physics, Dalian, 116023, China |
| Abstract: | The fluorescence of acridine orange (AO) can be quenched significantly with addition of the single-wall carbon nanotubes (SWCNTs) solution, due to the formation of a hybrid complex between AO and SWCNTs. A fluorescence enhancement of approximately 18× can be observed after the addition of certain amount of DNA into the above mentioned solution. The fluorescence increase was linearly proportional to the amount of DNA added in the concentration range of 0–50.75 μM, and the DNA detection limit was down to 8.56 × 10−8 M. This method can be used to detect DNA in vitro. |
| Keywords: | Single-wall carbon nanotubes (SWCNTs) Acridine orange (AO) Hybrid DNA Fluorescence detection |
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