| HPLC中蛋白质色谱柱键载量的测定 |
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| 引用本文： | 薛树科,敦慧娟.HPLC中蛋白质色谱柱键载量的测定[J].河北化工,2012,35(1):62-64,72. |
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| 作者姓名： | 薛树科 敦慧娟 |
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| 作者单位： | 1. 河北师范大学附属民族学院,河北石家庄,050091
2. 河北师范大学化学与材料科学学院,河北石家庄,050024 |
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| 摘 要： | 色谱柱键合量是对色谱柱进行表征的一个重要参数,而现有文献对于蛋白质色谱柱的键合量测定至今未见报道。首次建立了利用考马斯亮蓝方法测定蛋白质色谱柱键载量的一种分析方法。同时考察了最大吸收波长、酸度、考马斯亮蓝浓度、测定时间等对测定蛋白质键载量的影响。采用物理吸附和化学键合2种方法制备了2种牛血清白蛋白色谱柱,测定了其蛋白质键合量。
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| 关 键 词： | HPLC 蛋白质色谱柱 键载量 |
Detection for Load of Protein Chromatographic Colum in HPLC |
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| XUE Shu-ke,DUN Hui-juan.Detection for Load of Protein Chromatographic Colum in HPLC[J].Hebei Chemical Engineering and Industry,2012,35(1):62-64,72. |
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| Authors: | XUE Shu-ke DUN Hui-juan |
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| Affiliation: | 1.National Institute Affiliated of Hebei Normal University,Shijiazhuang 050091,China;2.College of Chemistry and Material Sciences of Hebei Normal University,Shijiazhuang 050024,China) |
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| Abstract: | Chromatographic column bonding of chromatographic column is one of the important parameters for characterization and the existing literature for protein chromatography column bonding quantity determination has not been seen.To establish a analysis method which using Coomassie brilliant blue to determine protein chromatography column bonding quantity,and study the influnce of some key aspects,such as maximum absorption wavelength,acidity,the concentration of Coomassie brilliant blue,stability on detection of load for protein.To research two kinds of methods for two bovine serum albumin chromatographic column based on physical adsorption and chemical preparation,and measure the protein bonding. |
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| Keywords: | HPLC protein chromatographic colum load |
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