| 普那霉素发酵工艺研究 |
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| 引用本文： | 钱思宇,刘颖利,王昂,周旻,郎晓磊,仓基勇. 普那霉素发酵工艺研究[J]. 河北化工, 2012, 35(4): 9-11 |
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| 作者姓名： | 钱思宇 刘颖利 王昂 周旻 郎晓磊 仓基勇 |
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| 作者单位： | 华北制药集团新药研究开发有限责任公司,河北省工业微生物代谢工程技术研究中心,河北石家庄050015 |
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| 摘 要： | 以RP59500为试验菌株,在15 L发酵罐内,研究了普那霉素的发酵工艺。通过单因素条件试验确定了其生物合成的最适温度、接种量。优化了其发酵培养基,并进行了发酵中间补料试验,确定了最佳补料工艺。试验结果表明：以3%淀粉、1.7%蔗糖、1%黄豆粉、1.5%酵母粉、0.5%葡萄糖、0.01%KH2PO4、0.2%MgSO4、0.2%泡敌和0.4%CaCO3的发酵培养基配方,以接种量4.0%,26℃下发酵,在发酵21 h时补加氨基酸,普那霉素的发酵效价达3 000 U/mL。
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| 关 键 词： | 普那霉素 发酵 工艺优化 补料 |
Research on the Fermentation Techology of Pristinamycin |
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| QIAN Si-yu,LIU Ying-li,WANG Ang,ZHOU Min,LANG Xiao-lei,CANG Ji-yong. Research on the Fermentation Techology of Pristinamycin[J]. Hebei Chemical Engineering and Industry, 2012, 35(4): 9-11 |
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| Authors: | QIAN Si-yu LIU Ying-li WANG Ang ZHOU Min LANG Xiao-lei CANG Ji-yong |
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| Affiliation: | (New Drugs of Research & Developing Center,North China Pharmaceutical Group Corporation,Hebei Industry Mi-crobial Metabolic Engineering & Technology Research Center,Shijiazhuang 050015,China) |
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| Abstract: | The fermentation techology of pristinamycin with RP59500 as experimental strains was studied in a 15 L fer-mentor.Through single-factor experiments,the optimum fermentation temperature,inoculation volume were obtained.The fermentation medium composition was optimized.The titer of pristinamycin increased greatly by using feeding fer-mentation.The results showed that the titer of pristinamycin reached 3 000 μ/mL with 4.0 % inoculation volume at 26 ℃.The optimal medium contains 3% starch,1.7% sucrose,1% soybean powder,1.5% yeast powder,0.5% glucose,0.01% KH2PO4,0.2% MgSO4,0.2% defoaming agent,0.4% CaCO3,and the amino acid was fed after it had been fermen-tated for 21 h. |
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| Keywords: | pristinamycin fermentation process condition optimization feeding |
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