薄层扫描法同时测定南、北五味子提取物中四种木脂素的含量

目的:建立同时测定南、北五味子提取物中四种主要木脂素含量的方法。方法:分别采用乙醇、超临界CO2提取南、北五味子中的木脂素,采用GF254板,展开剂为正己烷-醋酸乙酯(8:3),双波长薄层扫描(λS=220nm,λR=340nm)测定南、北五味子提取物中五味子醇甲、酯甲、甲素和乙素的含量。结果:五味子醇甲点样量于0.44～1.54μg,酯甲点样量于0.60～2.10μg,甲素点样量于0.52～1.82μg,乙素点样量于0.48～1.68μg范围内与峰面积呈良好线性关系,相关系数R分别为0.9986、0.9960、0.9997和0.9962,平均回收率分别为104.5%、95.0%、96.1%和102.1%,5次测定的RSD分别为4.0%、2.8%、2.6%和1.9%。超萃提取物中四种木脂素含量均高于醇提物。结论:本法简便、快速、精密度较好,可用于同时测定五味子提取物中四种主要木脂素含量。

Simultaneous Determination of the Four Lignans in Extract of Schisandra chinensis and S. sphenanthera by TLCS

Objective: To establish a method for determining the content of the four lignans in extract of Schisandra chinensis and S.sphenanthera simultaneously. MethodSamples were extracted with alcohol and CO2-SFE. The contents of schisandrin,Wuweizi ester A,schisandrin A and schisandrin B could be determined simultaneously by dual wavelength TLC- Scanning (λS=220nm,λR=340nm) after the extracts were developed by n-hexane- ethyl acetate (8:3) on self-made GF254 plates. ResultThe calibration curves were linear in range of 0.44～1.54μg for schisandrin(R=0.9986),0.60～2.10μg for Wuweizi ester A(R=0.9960),0.52～1.82μg for schisandrin A(R=0.9997) and 0.48 ～1.68μg for schisandrin B(R=0.9962) respectively. The average recoveries(n=5) were 104.5% for schisandrin(RSD=4.0%),95.0% for wuweizi ester A(RSD=2.8%),96.1% for schisandrin A (RSD=2.6%) and 102.1% for schisandrin B(RSD=1.9%). All of the contents of four lignans in extracts of schisandra with SCF- CO2 are higher than that in extracts of schisandra with alcohol. Conclusion: This method was found to be simple,fast and accurate for simultaneously determining the content of the four lignans in extract of Schisandra chinensisl and S. sphenanthera.