The effect of phospholipase D on the function of fragmented sarcoplasmic reticulum |
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Authors: | W Fiehn |
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Affiliation: | Medizinische Universitäts-Poliklinik, Heidelberg, 6900 Germany |
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Abstract: | Incubations of fresh preparations of fragmented sarcoplasmic reticulum (FSR) were carried out at pH 5.7. This pH was necessary
for hydrolysis of phospholipids by phospholipase D. The pH did not influence calcium uptake or the activity of calcium-stimulated
ATPase of FSR. Treatment of FSR with phospholipase D caused hydrolysis of the membrane phospholipids. The phosphatidic acid
produced remained bound to the membrane. Increasing phospholipid cleavage was paralleled by loss of calcium uptake, which
was complete when about two-thirds of the membrane phospholipids were hydrolyzed. |
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