姬松茸胞外多糖AbEXP1-a免疫调节活性研究

为研究姬松茸胞外多糖AbEXP1-a的免疫调节活性,采用昆明种小鼠以腹腔注射方式给药,对免疫器官质量、腹腔巨噬细胞吞噬鸡红细胞指数、鸡红细胞作免疫源的溶血素含量、小鼠外周血中淋巴细胞酶标记染色、2,4-二硝基氟苯诱发的迟发型变态反应等指标进行检测.给药剂量分别为每千克体重5 mg1、0 mg、30 mg.结果表明:多糖AbEXP1-a对小鼠非特异性免疫、体液免疫和细胞免疫均有明显的促进作用,可提高小鼠迟发性变态反应,即可以全面提高机体的免疫功能.多糖AbEXP1-a的最适作用剂量为每千克体重10 mg.

Study on Immunomodulating Activity of Exo-polysaccharide Extracted from Agaricus Blazei Broth

To research the immunomodulating activity of exo-polysaccharide AbEXP1-a extracted from Agaricus blazei,Kunming mice were used to detect AbEXP1-a dosages(5,10,30 mg/kg) on immune activities(i.p.).The measured items include immune organ weight,chicken red blood cell phagocytic index of macrophage cell of abdominal cavity,hemoglobin amount analysis with chicken red blood cell as immune source,ELISA of lymphocyte in mice peripheral blood and DNFB-induced DTH.Results are as follows: AbEXP1-a can increase spleen and thymus index,phagocytic function of macrophage cell and hemoglobin amount.And it can enhances DNFB-induced DTH in mice.AbEXP1-a has the ability to promote aspecific immunity,specific l immunity and cellular immunity in mice.