| 生化反应──色谱分离耦合过程模型研究 |
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| 引用本文： | 吴金川 何志敏. 生化反应──色谱分离耦合过程模型研究[J]. 化工学报, 1996, 47(2): 192-198 |
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| 作者姓名： | 吴金川 何志敏 |
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| 作者单位： | 天津大学化学工程研究所!天津300072,天津大学化学工程研究所!天津300072,天津大学化学工程研究所!天津300072 |
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| 基金项目： | 国家教委优秀年轻教师基金，重点跟踪资助 |
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| 摘 要： | 建立了描述生化反应─色谱分离耦合过程的一般数学模型，以蔗糖酶促水解制果糖、葡萄糖为模型反应，研究了底物转化率和产物分离度与操作条件的关系.理论计算值与实验结果在低浓度下符合较好．
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| 关 键 词： | 色谱分离 耦合过程 水解 生物化学反应 蔗糖酶 |
STUDY ON PROCESS OF SIMULTANEOUS BIOCHEMICAL REACTION AND CHROMATOGRAPHIC SEPARATION |
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| Wu Jinchuan,He Zhimin,Yu Guocong. STUDY ON PROCESS OF SIMULTANEOUS BIOCHEMICAL REACTION AND CHROMATOGRAPHIC SEPARATION[J]. Journal of Chemical Industry and Engineering(China), 1996, 47(2): 192-198 |
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| Authors: | Wu Jinchuan He Zhimin Yu Guocong |
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| Abstract: | A generalized mathematical model was developed to simulate the process of simultaneous biochemical reaction and chromatographic separation.The hydrolysis of sucrose to glucose and fructose was chosen as a model reaction to study the coupling process in a 150cm×1cm glass column.It was found that not only sucrose is completely hydrolyzed,but the glucose-rich and fructose-rich products are also separated.Compared to the concentration overload,the volume overload leads to a higher substrate conversion,while there is little difference for the resolution of products.A good agreement between the theoretical and experimental results is reached at the lower sucrose concentrations. |
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| Keywords: | biochemical reaction chromatographic separation coupling process sucrose hydrolysis |
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