烟草NtSAP5基因克隆及干旱胁迫下的功能鉴定

为研究烟草对干旱胁迫的响应机制，克隆了烟草中锌指蛋白基因NtSAP5并对其在干旱胁迫下的表达量进行了检测。结果表明，该基因全长CDS序列为474 bp，编码的蛋白中含有保守的锌指蛋白结构域，该基因NtSAP5的表达受干旱胁迫调控，在干旱胁迫下表达量先升高，后缓慢降低。将NtSAP5的CDS序列克隆到植物过表达载体pG2BW7中，并通过叶盘法转入烟草K326中进行干旱胁迫下的功能验证。选取两个独立的转基因株系及非转基因植株进行干旱胁迫处理，过表达NtSAP5的转基因植株比非转基因植株具有较强的抵御干旱胁迫能力。结果表明，烟草NtSPA5响应干旱胁迫，并在干旱胁迫应答中起到作用。 

Cloning of Tobacco NtSAP5 gene and functional identification under drought stress

To study the response mechanism of tobacco to drought stress, NtSAP5 gene of zinc finger protein in tobacco was cloned and its expression under drought stress was detected in this study. The full-length coding sequence (CDS) was 474 bp and the encoding protein contains conserved zinc finger domain. The expression of NtSAP5 was regulated by drought stress, and the expression level first increased and then decreased slowly under drought stres. The CDS sequence of NtSAP5 gene was cloned into plant overexpression vector pG2BW7 and transferred to tobacco variety K326 by leaf disc method for functional verification under drought stress. Two independent transgenic lines and one non-transgenic control line were treated by drought stress. The overexpressed lines exhibited stronger drought resistance than the control. Thus, NtSPA5 played an important role in drought stress response.